Human EPB41L1 (Band 4.1-like protein 1) ELISA Kit | EPB41L1 elisa kit

Human EPB41L1 (Band 4.1-like protein 1) ELISA Kit

Cat. Num. AAA7612324

• Gene Names: EPB41L1; 4.1N; MRD11
• Reactivity: Human
• Synonyms: EPB41L1 (Band 4.1-like protein 1); Human EPB41L1 (Band 4.1-like protein 1) ELISA Kit; Band 4.1-like protein 1; Erythrocyte membrane protein band 4.1-like 1; Neuronal protein 4.1; 4.1N; EPB41L1; KIAA0338; EPB41L1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of EPB41L1. No significant cross-reactivity or interference between EPB41L1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EPB41L1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 14916561
• NCBI GeneID: 2036
• UniProt Accession #: Q9H4G0; O15046; Q4VXM6; Q4VXM7; Q4VXM8; Q4VXN4; Q6ZT61; Q8IUU7; Q96CV5; Q96L65
• Molecular Weight: 78,831 Da
• NCBI Official Full Name: Band 4.1-like protein 1
• NCBI Official Synonym Full Names: erythrocyte membrane protein band 4.1-like 1
• NCBI Official Symbol: EPB41L1
• NCBI Official Synonym Symbols: 4.1N; MRD11
• NCBI Protein Information: band 4.1-like protein 1; neuronal protein 4.1; neuron-type nonerythroid protein 4.1
• UniProt Protein Name: Band 4.1-like protein 1
• Protein Family: Band 4.1-like protein
• UniProt Gene Name: EPB41L1
• UniProt Synonym Gene Names: KIAA0338; 4.1N
• UniProt Entry Name: E41L1_HUMAN

NCBI Description

Erythrocyte membrane protein band 4.1 (EPB41) is a multifunctional protein that mediates interactions between the erythrocyte cytoskeleton and the overlying plasma membrane. The protein encoded by this gene is a neuronally-enriched protein that is structurally similar to EPB41. The encoded protein binds and stabilizes D2 and D3 dopamine receptors at the neuronal plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

EPB41L1: May function to confer stability and plasticity to neuronal membrane via multiple interactions, including the spectrin-actin-based cytoskeleton, integral membrane channels and membrane-associated guanylate kinases. Defects in EPB41L1 are the cause of mental retardation autosomal dominant type 11 (MRD11). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20q11.2-q12

Cellular Component: cytoskeleton; extrinsic to membrane; plasma membrane; cytosol

Molecular Function: actin binding; structural molecule activity

Biological Process: synaptic transmission; cortical actin cytoskeleton organization and biogenesis

Disease: Mental Retardation, Autosomal Dominant 11