Mouse Hepatocyte Nuclear Factor 1 Alpha (HNF1a) CLIA Kit | HNF1a clia kit

Mouse Hepatocyte Nuclear Factor 1 Alpha (HNF1a) CLIA Kit

Cat. Num. AAA2001025

• Gene Names: HNF1A; HNF1; LFB1; TCF1; MODY3; TCF-1; HNF-1A; IDDM20
• Reactivity: Mouse
• Synonyms: Hepatocyte Nuclear Factor 1 Alpha (HNF1a); Mouse Hepatocyte Nuclear Factor 1 Alpha (HNF1a) CLIA Kit; TCF1; HNF1; LFB1; LF-B1; MODY3; Transcription Factor 1; Hepatic; Hepatic Nuclear Factor; Albumin Proximal Factor; Liver-specific transcription factor LF-B1; HNF1a clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hepatocyte Nuclear Factor 1 Alpha (HNF1a).; No significant cross-reactivity or interference between Hepatocyte Nuclear Factor 1 Alpha (HNF1a) and analogues was observed.
• Sequence Length: 108

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 13.7-10,000pg/mL
• Sensitivity: < 5.7pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Alpha (HNF1a) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Alpha (HNF1a) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HNF1a clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Alpha (HNF1a). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Alpha (HNF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepatocyte Nuclear Factor 1 Alpha (HNF1a) level in the sample or standard.;

Product Categories/Family for HNF1a clia kit

Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 219526070
• NCBI GeneID: 6927
• Molecular Weight: 11,300 Da
• NCBI Official Full Name: hepatocyte nuclear factor 1 alpha, partial
• NCBI Official Synonym Full Names: HNF1 homeobox A
• NCBI Official Symbol: HNF1A
• NCBI Official Synonym Symbols: HNF1; LFB1; TCF1; MODY3; TCF-1; HNF-1A; IDDM20
• NCBI Protein Information: hepatocyte nuclear factor 1-alpha
• UniProt Protein Name: Hepatocyte nuclear factor 1 alpha
• Protein Family: Hepatocyte nuclear factor
• UniProt Gene Name: HNF1alpha
• UniProt Entry Name: B8YNV0_HUMAN

NCBI Description

The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Uniprot Description

HNF1A: Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'- GTTAATNATTAAC-3'. Defects in HNF1A are a cause of hepatic adenomas familial (HEPAF). Hepatic adenomas are rare benign liver tumors of presumable epithelial origin that develop in an otherwise normal liver. Hepatic adenomas may be single or multiple. They consist of sheets of well-differentiated hepatocytes that contain fat and glycogen and can produce bile. Bile ducts or portal areas are absent. Kupffer cells, if present, are reduced in number and are non-functional. Conditions associated with adenomas are insulin-dependent diabetes mellitus and glycogen storage diseases (types 1 and 3). Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas. Defects in HNF1A are the cause of maturity-onset diabetes of the young type 3 (MODY3); also symbolized MODY-3. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Defects in HNF1A are the cause of susceptibility to diabetes mellitus insulin-dependent type 20 (IDDM20). IDDM20 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These features can result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Belongs to the HNF1 homeobox family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: nucleoplasm; protein complex; cytoplasm; nucleus

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; insulin secretion; positive regulation of transcription, DNA-dependent; glucose import; positive regulation of transcription from RNA polymerase II promoter; glucose homeostasis

Disease: Diabetes Mellitus, Insulin-dependent, 20; Diabetes Mellitus, Insulin-dependent; Hepatic Adenomas, Familial; Maturity-onset Diabetes Of The Young, Type 3; Renal Cell Carcinoma, Nonpapillary; Diabetes Mellitus, Noninsulin-dependent