Mouse Epithelial Neutrophil Activating Peptide 78 (ENA78) CLIA Kit | ENA78 clia kit

Mouse Epithelial Neutrophil Activating Peptide 78 (ENA78) CLIA Kit

Cat. Num. AAA2708873

• Gene Names: Cxcl5; LIX; Cxcl6; GCP-2; Scyb5; Scyb6; ENA-78; AMCF-II
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Epithelial Neutrophil Activating Peptide 78 (ENA78); Mouse Epithelial Neutrophil Activating Peptide 78 (ENA78) CLIA Kit; Epithelial Neutrophil Activating Peptide 78; CXCL5; SCYB5; Chemokine C-X-C-Motif Ligand 5; Small Inducible Cytokine Subfamily B(Cys-X-Cys); Member 5; Neutrophil-activating peptide ENA-78; ENA78 clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Epithelial Neutrophil Activating Peptide 78 (ENA78).; No significant cross-reactivity or interference between Epithelial Neutrophil Activating Peptide 78 (ENA78) and analogues was observed.
• Sequence Length: 132

• Applicable Applications for ENA78 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.52pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epithelial Neutrophil Activating Peptide 78 (ENA78) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epithelial Neutrophil Activating Peptide 78 (ENA78) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Epithelial Neutrophil Activating Peptide 78 (ENA78). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Epithelial Neutrophil Activating Peptide 78 (ENA78). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Epithelial Neutrophil Activating Peptide 78 (ENA78) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 114842398
• NCBI GeneID: 20311
• NCBI Accession #: NP_033167.2
• NCBI GenBank Nucleotide #: NP_033167.2
• UniProt Accession #: P50228
• NCBI Official Full Name: C-X-C motif chemokine 5
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 5
• NCBI Official Symbol: Cxcl5
• NCBI Official Synonym Symbols: LIX; Cxcl6; GCP-2; Scyb5; Scyb6; ENA-78; AMCF-II
• NCBI Protein Information: C-X-C motif chemokine 5
• UniProt Protein Name: C-X-C motif chemokine 5
• UniProt Gene Name: Cxcl5
• UniProt Synonym Gene Names: Scyb5
• UniProt Entry Name: CXCL5_MOUSE

NCBI Description

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils and to have homeostatic and inflammatory functions. In mouse, deficiency of this gene is associated with increased lung inflammation that is neutrophil-dependent. [provided by RefSeq, May 2013]

Uniprot Description

CXCL6: Chemotactic for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: extracellular space

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: cytokine production; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; leukocyte homeostasis; organ regeneration; positive regulation of JAK-STAT cascade; positive regulation of leukocyte chemotaxis; regulation of cell proliferation; regulation of chemokine production; response to lipopolysaccharide