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Typical Testing Data/Standard Curve (for reference only)

Mouse Elastin Microfibril Interface Located Protein 2 (EMILIN2) CLIA Kit | EMILIN2 clia kit

Mouse Elastin Microfibril Interface Located Protein 2 (EMILIN2) CLIA Kit

Reactivity
Mouse
Applications
Chemiluminescence Immunoassay (CLIA)
Synonyms
Elastin Microfibril Interface Located Protein 2 (EMILIN2); Mouse Elastin Microfibril Interface Located Protein 2 (EMILIN2) CLIA Kit; Elastin Microfibril Interface Located Protein 2; FOAP-10; Elastin Microfibril Interfacer 2; EMILIN2 clia kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Elastin Microfibril Interface Located Protein 2 (EMILIN2).
No significant cross-reactivity or interference between Elastin Microfibril Interface Located Protein 2 (EMILIN2) and analogues was observed.
Sequence Length
1074
Applicable Applications for EMILIN2 clia kit
Chemiluminescence Immunoassay (CLIA)
Assay Type
Double-antibody Sandwich
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
13.7-10,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 4.8pg/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Elastin Microfibril Interface Located Protein 2 (EMILIN2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Elastin Microfibril Interface Located Protein 2 (EMILIN2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Elastin Microfibril Interface Located Protein 2 (EMILIN2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Elastin Microfibril Interface Located Protein 2 (EMILIN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Elastin Microfibril Interface Located Protein 2 (EMILIN2) level in the sample or standard.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
EMILIN-2 isoform 1
UniProt Protein Name
EMILIN-2
Protein Family
UniProt Gene Name
Emilin2
UniProt Synonym Gene Names
Elastin microfibril interfacer 2

Uniprot Description

May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity. Major component of the cochlear basilar membrane (BM) which may contribute to the developmental assembly or function of the BM.

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Product Notes

The Mouse EMILIN2 emilin2 (Catalog #AAA2709202) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709202 CLIA Kit recognizes Mouse EMILIN2. AAA Biotech's Elastin Microfibril Interface Located Protein 2 (EMILIN2) can be used in a range of immunoassay formats including, but not limited to, Chemiluminescence Immunoassay (CLIA). Researchers should empirically determine the suitability of the EMILIN2 emilin2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Elastin Microfibril Interface Located Protein 2 (EMILIN2), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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