Mouse Collagen Type IV Antibody | anti-COL4A1 antibody

Collagen Type IV, human

Cat. Num. AAA397110

• Gene Names: COL4A1; BSVD; RATOR
• Reactivity: Human. Other primates. No reactivity in Chicken, Cat, Rabbit, Rat, Sheep and Goat.
• Applications: Immunohistochemistry, Western Blot
• Synonyms: Collagen Type IV; human; anti-COL4A1 antibody

• Host: Mouse
• Reactivity: Human. Other primates. No reactivity in Chicken, Cat, Rabbit, Rat, Sheep and Goat.
• Isotype: IgG1
• Clone Number: COL 94
• Form/Format: Solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide.
• Concentration: 100 ug/ml (varies by lot)
• Sequence Length: 519

• Applicable Applications for anti-COL4A1 antibody: Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB)
• Application Notes: Dilution: 1:10-1:50
• Immunogen: Purified human Collagen IV
• Positive Control: Human skin, placenta or lens capsule
• Incubation Time: 60 min at RT
• Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for anti-COL4A1 antibody

Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human Collagen IV, no cross-reactivity with other Collagen types, Vitronectin, Fibronectin or Chondroitinsulfate.

References

1. Odermatt B.F., Lang A.B., Rüttner J.R., Winterhalter K.H., and Trüeb B. (1984) Monoclonal antibodies to human type IV collagen: useful reagents to demonstate the heterotrimeric nature of the molecule. Proc. Natl. Acad. Sci. 81; 7343-7347.

NCBI and Uniprot Product Information

• NCBI GI #: 734520332
• NCBI GeneID: 1282
• NCBI Accession #: NP_001290039.1
• NCBI GenBank Nucleotide #: NM_001303110.1
• UniProt Accession #: P02462; Q1P9S9; Q5VWF6; Q86X41; Q8NF88; Q9NYC5; A7E2W4; B1AM70; F5H5K0
• Molecular Weight: 50,155 Da
• NCBI Official Full Name: collagen alpha-1(IV) chain isoform 2
• NCBI Official Synonym Full Names: collagen type IV alpha 1 chain
• NCBI Official Symbol: COL4A1
• NCBI Official Synonym Symbols: BSVD; RATOR
• NCBI Protein Information: collagen alpha-1(IV) chain
• UniProt Protein Name: Collagen alpha-1(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: COL4A1

NCBI Description

This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.