Mouse Neurofilaments Antibody
Neurofilaments 70/200 kDa
Reactivity
Human, Cattle, Dog, Horse, Mouse, Pig, Rat
Applications
Immunohistochemistry, Immunoblot, Western Blot
Synonyms
Neurofilaments; Neurofilaments 70/200 kDa; anti-Neurofilaments antibody
Host
Mouse
Reactivity
Human, Cattle, Dog, Horse, Mouse, Pig, Rat
Isotype
IgG1
Clone Number
2F11
Form/Format
Solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide.
Applicable Applications for anti-Neurofilaments antibody
Immunohistochemistry (IHC) Formalin/Paraffin, Immunoblotting (IB)
Application Notes
Dilution: 1:10-1:50
Immunogen
Purified neurofilament proteins from human brain
Positive Control
Nerve tissue
Incubation Time
60 min at RT
Pre-Treatment
No protease pre-treatment required! Blocking of endogenous biotin and alkaline phosphatase is recommended, if avidin/biotin systems are used.
Pre-Treatment
No protease pre-treatment required! Blocking of endogenous biotin and alkaline phosphatase is recommended, if avidin/biotin systems are used.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for anti-Neurofilaments antibody
This antibody may also stain phaeochromocytoma and medullary adrenal cells. Neurofilament proteins are characteristic intermediary filaments of neurons. They are divided into three classes of molecular weights: 70, 160 and 200 kDa. As well central as peripheral nerve fibres are stained with this antibody. Glia cells are antibody negative. Neurofilaments 70 and 200 kDa.
References
1. Osborn M., Altmannsberger M., Shaw G., Schauer A., Weber K. (1982) Various sympathetic derived human tumors differ in neurofilament expression. Use in Diagnosis of neuroblastoma, ganglioneuroblastoma and pheochromocytoma. Virchows Arch. Cell Pathol. 40; 141-156. 2. Alfonsi F., Darmon M., Forest N., and Paulin D. (1983) Intermediate sized filaments as markers of neuronal differentiation in the role of cell interaction in early neurogenesis. In: Plenum Publishing Corporation A.M. Duprat, A. Kato, and M. Weber eds. pp 157-176. 3. Osborn M., and Weber K. (1983) Biology of disease tumor diagnosis by intermediate filament typing. Lab. Invest. 48; 372-394. 4. Klück P., et al. (1984) Hirschsprung disease studied with monoclonal anti neurofilament antibodies on tissue sections. The Lancet, March 24, 652-653. 5. Osborn M., Dirk T., Käser H., Weber K., and Altmannsberger M. (1986) Immunohistochemical localization of neurofilaments and neuron-specific enolase in 29 cases of neuroblastoma. Am. J. Pathol. 122; 433-442.
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Product Notes
The Neurofilaments (Catalog #AAA397290) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Neurofilaments 70/200 kDa reacts with Human, Cattle, Dog, Horse, Mouse, Pig, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofilaments can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin/Paraffin, Immunoblotting (IB). Dilution: 1:10-1:50. Researchers should empirically determine the suitability of the Neurofilaments for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurofilaments, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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