Western Blot (WB)
(All lanes: Recombinant GST Protein at 30ng/mlLine 1:Mouse Anti-GST monoclonal antibody at 1:1000Line2:Mouse Anti-GST monoclonal antibody at 1:2000Line3:Mouse Anti-GST monoclonal antibody at 1:5000Line4:Mouse Anti-GST monoclonal antibody at 1:10000Line5:Mouse Anti-GST monoclonal antibody at 1:20000Line6:Mouse Anti-GST monoclonal antibody at 1:40000Line7:Mouse Anti-GST monoclonal antibody at 1:80000Predicted band size: 28kdObserved band size: 30kd)
Mouse GST Antibody | anti-GST antibody
Mouse Anti-GST monoclonal Antibody
Applications
ELISA, Western Blot
Purity
>95%, Protein G Purified
Synonyms
GST; Mouse Anti-GST monoclonal Antibody; Glutathione S-Transferase; gst; GST tag; anti-GST antibody
Host
Mouse
Isotype
IgG2a
Purity/Purification
>95%, Protein G Purified
Applicable Applications for anti-GST antibody
ELISA (EIA), Western Blot (WB)
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant GST Protein
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(All lanes: Recombinant GST Protein at 30ng/mlLine 1:Mouse Anti-GST monoclonal antibody at 1:1000Line2:Mouse Anti-GST monoclonal antibody at 1:2000Line3:Mouse Anti-GST monoclonal antibody at 1:5000Line4:Mouse Anti-GST monoclonal antibody at 1:10000Line5:Mouse Anti-GST monoclonal antibody at 1:20000Line6:Mouse Anti-GST monoclonal antibody at 1:40000Line7:Mouse Anti-GST monoclonal antibody at 1:80000Predicted band size: 28kdObserved band size: 30kd)
Western Blot (WB)
(All lanes: Recombinant GST Protein at 30ng/mlLine 1:Mouse Anti-GST monoclonal antibody at 1:1000Line2:Mouse Anti-GST monoclonal antibody at 1:2000Line3:Mouse Anti-GST monoclonal antibody at 1:5000Line4:Mouse Anti-GST monoclonal antibody at 1:10000Line5:Mouse Anti-GST monoclonal antibody at 1:20000Line6:Mouse Anti-GST monoclonal antibody at 1:40000Line7:Mouse Anti-GST monoclonal antibody at 1:80000Predicted band size: 28kdObserved band size: 30kd)
Related Product Information for anti-GST antibody
Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the DYKDDDDK-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
Product Categories/Family for anti-GST antibody
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Product Notes
The GST (Catalog #AAA1497871) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GST can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the GST for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GST, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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