Thrombospondin Type-1 Domain-containing Protein 1 Active Protein | THSD1 active protein

Thrombospondin Type-1 Domain-containing Protein 1, Recombinant, Mouse (THSD1, Transmembrane Molecule with Thrombospondin Module, Tmtsp)

Cat. Num. AAA638145

• Gene Names: THSD1; TMTSP; UNQ3010
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Highly Purified; 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1EU/ug protein (LAL)
• Synonyms: Thrombospondin Type-1 Domain-containing Protein 1; Recombinant; Mouse (THSD1; Transmembrane Molecule with Thrombospondin Module; Tmtsp); THSD1 active protein

• Reactivity: Mouse
• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1EU/ug protein (LAL)
• Form/Format: Supplied as a lyophilized powder from PBS. Reconstitute with 500ul sterile PBS.

• Applicable Applications for THSD1 active protein: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 44.3kD migrates as an ~70-78kD protein in SDS-PAGE under reducing conditions.
• Activity: Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. When 5x10e4 cells/well of are added to recombinant mouse THSD1 coated plates (30ug/ml/well) ~50-80% will adhere after 60 minutes at 37ºC
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for THSD1 active protein

Mouse THSD1 (Thrombospondin type-1 domain-containing protein 1), also known as Transmembrane molecule with thrombospondin module (Tmtsp), is a 95kD, type I transmembrane protein (1). It is synthesized as a precursor that is 851 amino acids (aa) in length, and has a 24 aa signal sequence, a 388 aa extracellular region, a 21 aa transmembrane region, and a 418 aa cytoplasmic region. The extracellular region contains six potential N-linked glycosylation sites and the thrombospondin type-1 (TSP1) domain, which is implicated in cell adhesion and migration (1). Also present in the molecule are 9 PKC-phosphorylation sites, 3 Ig-like domains, and a tyrosine-phosphorylation site by its C-terminus (1). Mouse THSD1 shares 78% aa sequence identity with human THSD1. THSD1 is highly expressed in hematopoietic stem cells (HSCs) and progenitor cells, including CD34-/lowKit+Sca-1+Lin- HSCs, CD34+Kit+Sca-1+Lin- and Lin-progenitor cells, and to a lesser degree in thymic CD4-CD8- cells (1). Expression gradually decreases during T cell differentiation (1). In addition, THSD1 is widely expressed on endothelial cells, with highest expression in the lung (1). THSD1's ligand and function are unknown, but it is postulated that THSD1 may be involved in the regulation of vasculogenesis and/or angiogenesis through its interaction with its specific ligand (1). THSD1 may function in primitive hematopoietic cells in the bone marrow niche, and through its TSP1 and Ig-like domains, may play a role in HSC homing to the niche following cell-to-cell contact to maintain hematopoietic stem cell quiescence (1).

Product Categories/Family for THSD1 active protein

Molecular Biology; MB-Thrombospondin

NCBI and Uniprot Product Information

• NCBI GI #: 8923894
• NCBI GeneID: 55901
• NCBI Accession #: NP_061146.1
• NCBI GenBank Nucleotide #: NM_018676.3
• UniProt Accession #: Q9NS62; Q6P3U1; Q6UXZ2; A2A3J3; B2RCF5
• Molecular Weight: 44.3kD
• NCBI Official Full Name: thrombospondin type-1 domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: thrombospondin, type I, domain containing 1
• NCBI Official Symbol: THSD1
• NCBI Official Synonym Symbols: TMTSP; UNQ3010
• NCBI Protein Information: thrombospondin type-1 domain-containing protein 1; 4833423O18Rik; thrombospondin, type I, domain 1; transmembrane molecule with thrombospondin module
• UniProt Protein Name: Thrombospondin type-1 domain-containing protein 1
• Protein Family: Thrombospondin type-1 domain-containing protein
• UniProt Gene Name: THSD1
• UniProt Synonym Gene Names: TMTSP
• UniProt Entry Name: THSD1_HUMAN

NCBI Description

The protein encoded by this gene contains a type 1 thrombospondin domain, which is found in a number of proteins involved in the complement pathway, as well as in extracellular matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

THSD1: contains a type 1 thrombospondin domain, which is found in a number of proteins involved in the complement pathway, as well as in extracellular matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Jan 2009]

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: cell surface; cytoplasm; extracellular region; integral to membrane

Biological Process: hemopoietic progenitor cell differentiation