Immunocytochemistry (ICC)
(Immunocytochemistry staining of capacitated boar sperm. Acrosome visualized using mouse monoclonal ACR-2 (purified; secondary antibody labeled with FITC, green), DNA stained with DAPI (blue).)
Mouse anti-Pig Por Acrosin Antibody | anti-ACR antibody
Anti-Por Acrosin Purified
Reactivity
Pig
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Western Blot
Purity
Purified by Protein-A Affinity Chromatography
Synonyms
Por Acrosin; Anti-Por Acrosin Purified; acrosin; ACR; 53 kDa fucose-binding protein; anti-ACR antibody
Host
Mouse
Reactivity
Pig
Isotype
IgG1
Clone Number
ACR-2
Specificity
The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
Purity/Purification
Purified by Protein-A Affinity Chromatography
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
400
Applicable Applications for anti-ACR antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
FC/FACS: QC tested
Immunogen
Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
Negative Species
Dog, Cow, Human
Preparation and Storage
Store at 2-8 degree C.
Immunocytochemistry (ICC)
(Immunocytochemistry staining of capacitated boar sperm. Acrosome visualized using mouse monoclonal ACR-2 (purified; secondary antibody labeled with FITC, green), DNA stained with DAPI (blue).)
Immunocytochemistry (ICC)
(Immunocytochemistry staining of capacitated boar sperm. Acrosome visualized using mouse monoclonal ACR-2 (purified; secondary antibody labeled with FITC, green), DNA stained with DAPI (blue).)
Related Product Information for anti-ACR antibody
Acrosin is a serine proteinase expressed in the acrosome of mature spermatozoa. This enzyme facilitates penetration of the sperm through the zona pellucida of the ovum.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
acrosin
NCBI Official Symbol
ACR
NCBI Protein Information
acrosin
UniProt Protein Name
Acrosin
Protein Family
UniProt Gene Name
ACR
Uniprot Description
Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.
Research Articles on ACR
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Product Notes
The ACR acr (Catalog #AAA1800323) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Por Acrosin Purified reacts with Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Por Acrosin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Western Blot (WB). FC/FACS: QC tested. Researchers should empirically determine the suitability of the ACR acr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Por Acrosin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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