Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ACOT2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Rabbit ACOT2 Polyclonal Antibody | anti-ACOT2 antibody
ACOT2 Polyclonal Antibody
Gene Names
ACOT2; MTE1; PTE2; CTE1A; PTE2A; CTE-IA; ZAP128
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Synonyms
ACOT2; Polyclonal Antibody; ACOT2 Polyclonal Antibody; CTE-IA; CTE1A; MTE1; PTE2; PTE2A; ZAP128; anti-ACOT2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
IVDMFGTGGGLLEYRASLLAGKGFAVMALAYYNYEDLPKTMETLHLEYFEEAMNYLLSHPEVKGPGVGLLGISKGGELCLSMASFLKGITAAVVINGSVANVGGTLHYKGETLPPVGVNRNRIKVTKDGYADIVDVLNSPLEGPDQKSFIPVERAESTFLFLVGQDDHNWKSEFYANEACKRLQAHGRRKPQIICYPETGHYIEPPYFPLCRASLHALVGSPIIWGGEPRAHAMAQVDAWKQLQTFFHKHLGGHE
Sequence Length
483
Applicable Applications for anti-ACOT2 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human ACOT2
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Mitochondrion
Positive Samples
293T, LO2, U-87MG, Mouse kidney
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ACOT2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ACOT2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Related Product Information for anti-ACOT2 antibody
This gene encodes a member of the acyl-CoA thioesterase protein family, and is one of four acyl-CoA hydrolase genes located in a cluster on chromosome 14. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-ACOT2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 32kDa; 53kDa
Observed: 50kDa
Observed: 50kDa
NCBI Official Full Name
acyl-coenzyme A thioesterase 2, mitochondrial
NCBI Official Synonym Full Names
acyl-CoA thioesterase 2
NCBI Official Symbol
ACOT2
NCBI Official Synonym Symbols
MTE1; PTE2; CTE1A; PTE2A; CTE-IA; ZAP128
NCBI Protein Information
acyl-coenzyme A thioesterase 2, mitochondrial
UniProt Protein Name
Acyl-coenzyme A thioesterase 2, mitochondrial
Protein Family
UniProt Gene Name
ACOT2
UniProt Synonym Gene Names
PTE2; PTE2A; Acyl-CoA thioesterase 2
NCBI Description
This gene encodes a member of the acyl-CoA thioesterase protein family, and is one of four acyl-CoA hydrolase genes located in a cluster on chromosome 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Uniprot Description
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Displays high levels of activity on medium- and long chain acyl CoAs.
Research Articles on ACOT2
Similar Products
Product Notes
The ACOT2 acot2 (Catalog #AAA9134395) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACOT2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ACOT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the ACOT2 acot2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: IVDMFGTGGG LLEYRASLLA GKGFAVMALA YYNYEDLPKT METLHLEYFE EAMNYLLSHP EVKGPGVGLL GISKGGELCL SMASFLKGIT AAVVINGSVA NVGGTLHYKG ETLPPVGVNR NRIKVTKDGY ADIVDVLNSP LEGPDQKSFI PVERAESTFL FLVGQDDHNW KSEFYANEAC KRLQAHGRRK PQIICYPETG HYIEPPYFPL CRASLHALVG SPIIWGGEPR AHAMAQVDAW KQLQTFFHKH LGGHE. It is sometimes possible for the material contained within the vial of "ACOT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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