Mouse XPA Monoclonal Antibody | anti-XPA antibody

XPA (Xeroderma Pigmentosum, Complementation Group A, XP1, XPAC) (APC)

Cat. Num. AAA6169951

• Gene Names: XPA; XP1; XPAC
• Applications: Immunofluorescence, Western Blot
• Purity: Purified
• Synonyms: XPA; Monoclonal Antibody; XPA (Xeroderma Pigmentosum; Complementation Group A; XP1; XPAC) (APC); Xeroderma Pigmentosum; XPAC; anti-XPA antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 20000
• Specificity: Recognizes XPA.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-XPA antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: XPA (NP_000371.1, 1aa-273aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: APC
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of XPA expression in transfected 293T cell line by XPA monoclonal antibody (M01), clone 2E4.Lane 1: XPA transfected lysate (Predicted MW: 31.4 KDa).Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged XPA is 0.3 ng/ml as a capture antibody.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to XPA on HeLa cell. [antibody concentration 10 ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to XPA on HeLa cell. [antibody concentration 10 ug/ml])

Related Product Information for anti-XPA antibody

This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Product Categories/Family for anti-XPA antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 4507937
• NCBI GeneID: 7507
• NCBI Accession #: NP_000371.1
• NCBI GenBank Nucleotide #: NM_000380.3
• UniProt Accession #: P23025; Q5T1U9; Q6LCW7; Q6LD02
• Molecular Weight: 31,368 Da
• NCBI Official Full Name: DNA repair protein complementing XP-A cells
• NCBI Official Synonym Full Names: xeroderma pigmentosum, complementation group A
• NCBI Official Symbol: XPA
• NCBI Official Synonym Symbols: XP1; XPAC
• NCBI Protein Information: DNA repair protein complementing XP-A cells; excision repair-controlling; xeroderma pigmentosum group A-complementing protein
• UniProt Protein Name: DNA repair protein complementing XP-A cells
• Protein Family: DNA repair protein
• UniProt Gene Name: XPA
• UniProt Synonym Gene Names: XPAC
• UniProt Entry Name: XPA_HUMAN

NCBI Description

This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

XPA: Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. Interacts with GPN1 and RPA1. Interacts (via N-terminus) with CEP164 upon UV irradiation. Interacts with HERC2. Expressed in various cell lines and in skin fibroblasts. Belongs to the XPA family.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: nucleoplasm; Golgi apparatus; intercellular bridge; DNA replication factor A complex; cytoplasm; nucleus

Molecular Function: protein domain specific binding; protein binding; protein homodimerization activity; metal ion binding; damaged DNA binding

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; nucleotide-excision repair; response to toxin; multicellular organism growth; nucleotide-excision repair, DNA damage removal; response to oxidative stress; DNA repair; response to UV

Disease: Xeroderma Pigmentosum, Complementation Group A