Immunofluorescence (IF)
(WRN antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with WRN antibody at a 0.5 ug/ml dilution.)
Mouse anti-Human WRN Monoclonal Antibody | anti-WRN antibody
WRN antibody (mAb)(Clone 195C)
ICC/IF: 0.5ug/ml dilution
IHC(P): User optimization required
WB: 0.5-2ug/ml dilution
Immunofluorescence (IF)
(WRN antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with WRN antibody at a 0.5 ug/ml dilution.)
Immunofluorescence (IF)
(WRN antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with WRN antibody at a 0.5 ug/ml dilution.)
Immunohistochemistry (IHC)
(WRN antibody (mAb) tested by Immunohistochemistry. Paraffin embedded tissue samples: human tonsil, MDA-MB-231 (breast adenocarcinoma cell line), AG11395 WS (fibroblast of a patient with Werner Syndrome immortalized with SV40), HeLa cells, MRC-5 (lung fibroblast cell line), and MCF-7 (breast carcinoma cell line).)
Immunohistochemistry (IHC)
(WRN antibody (mAb) tested by Immunohistochemistry. Paraffin embedded tissue samples: human tonsil, MDA-MB-231 (breast adenocarcinoma cell line), AG11395 WS (fibroblast of a patient with Werner Syndrome immortalized with SV40), HeLa cells, MRC-5 (lung fibroblast cell line), and MCF-7 (breast carcinoma cell line).)
Western Blot (WB)
(WRN antibody (mAb) tested by Western blot. Detection of WRN by Western blot. Lane 1: HeLa cell extract. Lane 2: WS cell extract (fibroblast from a patient with Werner Syndrome).)
Western Blot (WB)
(WRN antibody (mAb) tested by Western blot. Detection of WRN by Western blot. Lane 1: HeLa cell extract. Lane 2: WS cell extract (fibroblast from a patient with Werner Syndrome).)
Background: WRN protein is a RECQ helicase that contains 3' to 5' helicase activity and DNA-dependent ATPase activity. WRN takes part in many processes, including DNA replication, recombination, repair, and telomere maintenance. Mutations in the gene encoding WRN cause an autosomal recessive segmental progeria called Werner syndrome (WS). WS patients prematurely develop atrophic skin, thin gray hair, osteoporosis, type II diabetes, cataracts, arteriosclerosis and cancer.
NCBI and Uniprot Product Information
Uniprot Description
WRN: Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily.
Protein type: Nucleolus; EC 3.6.4.12; DNA repair, damage; Helicase; DNA-binding; Cell cycle regulation
Chromosomal Location of Human Ortholog: 8p12
Cellular Component: nucleoplasm; MutLalpha complex; centrosome; nucleolus
Molecular Function: G-quadruplex DNA binding; protein homodimerization activity; ATPase activity; magnesium ion binding; 3'-5' DNA helicase activity; bubble DNA binding; helicase activity; four-way junction helicase activity; ATP-dependent DNA helicase activity; Y-form DNA binding; protein binding; DNA helicase activity; DNA binding; ATP-dependent 3'-5' DNA helicase activity; manganese ion binding; exonuclease activity; protein complex binding; 3'-5' exonuclease activity; ATP binding
Biological Process: nucleolus to nucleoplasm transport; positive regulation of hydrolase activity; multicellular organismal aging; cell aging; cellular response to starvation; replicative cell aging; response to UV-C; DNA duplex unwinding; replication fork processing; DNA recombination; regulation of apoptosis; DNA synthesis during DNA repair; base-excision repair; double-strand break repair; regulation of growth rate; response to oxidative stress; DNA replication; response to DNA damage stimulus; DNA metabolic process; telomere maintenance; aging
Disease: Werner Syndrome
Similar Products
Product Notes
The WRN wrn (Catalog #AAA388792) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The WRN antibody (mAb)(Clone 195C) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WRN can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). Applications Validated: ICC/IF: 0.5ug/ml dilution IHC(P): User optimization required WB: 0.5-2ug/ml dilution. Researchers should empirically determine the suitability of the WRN wrn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WRN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
