Mouse West Nile Virus (WNV) E/Envelope Monoclonal Antibody | anti-WNV-prM antibody
West Nile Virus (WNV) (lineage 1, strain NY99) E/Envelope (Domain III) Antibody, Mouse MAb
Reactivity
West Nile Virus
Applications
ELISA
Purity
Protein A
Synonyms
West Nile Virus (WNV) E/Envelope; Monoclonal Antibody; West Nile Virus (WNV) (lineage 1; strain NY99) E/Envelope (Domain III) Antibody; Mouse MAb; Anti-WNV-prM and E Antibody; anti-WNV-prM antibody
Host
Mouse
Reactivity
West Nile Virus
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
03
Specificity
West Nile Virus (WNV) (lineage 1, strain NY99) E/Envelope (Domain III)
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-WNV-prM antibody
ELISA (EIA)
Application Notes
ELISA: 1:1000-1:2000
Antibody's applications haven't been validated with corresponding virus positive samples.
Antibody's applications haven't been validated with corresponding virus positive samples.
Immunogen
Recombinant West Nile Virus (WNV) (lineage 1, strain NY99) E/Envelope (Domain III)
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant West Nile Virus (WNV) (lineage 1, strain NY99) E/Envelope (Domain III). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Related Product Information for anti-WNV-prM antibody
Flaviviruses are a group of positive-stranded RNA viruses. West Nile virus (WNV) is a mosquito-borne neurotropic member of this genus that is an emerging pathogen. The envelope (E) protein is an approximately 53-kDa protein that mediates virus attachment to cells and drives the pH-dependent fusion of viral and cellular membranes in the endosomes of infected cells. On immature virions, the 20-kDa premembrane (prM) protein interacts with the E protein to form 60 heterotrimeric spikes and functions to prevent adventitious fusion between viral and cellular membranes during egress through acidic compartments of the secretory pathway. Transit through these compartments catalyzes a pH-dependent rearrangement of prM and E proteins on the virion that exposes a recognition motif on prM for cellular furin-like serine proteases.
References
Setoh YX, et al. (2012) Identification of residues in west nile virus pre-membrane protein that influence viral particle secretion and virulence. J Gen Virol 93 (Pt 9): 1965-1975.Mukherjee S, et al. (2011) The infectivity of prm-containing partially mature west nile virus does not require the activity of cellular furin-like proteases. J Virol 85 (22): 12067-12072.Calvert AE, et al. (2012) Mutations in the west nile prm protein affect vlp and virion secretion in vitro. Virology 433 (1): 35-44.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The WNV-prM (Catalog #AAA8110672) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The West Nile Virus (WNV) (lineage 1, strain NY99) E/Envelope (Domain III) Antibody, Mouse MAb reacts with West Nile Virus and may cross-react with other species as described in the data sheet. AAA Biotech's West Nile Virus (WNV) E/Envelope can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1:1000-1:2000 Antibody's applications haven't been validated with corresponding virus positive samples. Researchers should empirically determine the suitability of the WNV-prM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "West Nile Virus (WNV) E/Envelope, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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