Mouse anti-Human VTCN1 Monoclonal Antibody | anti-VTCN1 antibody

VTCN1 (V-set Domain Containing T-cell Activation Inhibitor 1, VCTN1, B7H4, B7-H4, B7h.5, B7S1, B7X, FLJ22418, Immune Costimulatory Protein B7-H4, PRO1291, Protein B7S1, RP11-229A19.4, T-cell Costimulatory Molecule B7x, UNQ659)

Cat. Num. AAA641175

• Gene Names: VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: VTCN1; Monoclonal Antibody; VTCN1 (V-set Domain Containing T-cell Activation Inhibitor 1; VCTN1; B7H4; B7-H4; B7h.5; B7S1; B7X; FLJ22418; Immune Costimulatory Protein B7-H4; PRO1291; Protein B7S1; RP11-229A19.4; T-cell Costimulatory Molecule B7x; UNQ659); Anti -VTCN1 (V-set Domain Containing T-cell Activation Inhibitor 1; anti-VTCN1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: H74
• Specificity: Recognizes human VTCN1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 150mM sodium chloride, 0.09% sodium azide.

• Applicable Applications for anti-VTCN1 antibody: Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry and Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 20ug/ml
• Immunogen: Human VTCN1
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

Testing Data

(Staining of non-transfected 293T cells with 0.125ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.125ug of Purified anti-human(colored histogram) followed by Biotin Anti-Mouse IgG and SAv-PE. Total cells were used for analysis.)

Testing Data

(Staining of human B7-H4 transfected 293T cells with 0.125ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.125ug of Purified anti-human(colored histogram) followed by Biotin Anti-Mouse IgG and SAv-PE. Total cells were used for analysis.)

Related Product Information for anti-VTCN1 antibody

Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.

Product Categories/Family for anti-VTCN1 antibody

Antibodies; Abs to Membrane Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 41350862
• NCBI GeneID: 79679
• UniProt Accession #: Q7Z7D3; Q0GN76; Q45VN0; Q5WPZ3; Q6P097; Q9H6B2
• Molecular Weight: 30,878 Da
• NCBI Official Full Name: VTCN1 protein
• NCBI Official Synonym Full Names: V-set domain containing T cell activation inhibitor 1
• NCBI Official Symbol: VTCN1
• NCBI Official Synonym Symbols: B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4
• NCBI Protein Information: V-set domain-containing T-cell activation inhibitor 1; B7 family member, H4; B7 superfamily member 1; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4
• UniProt Protein Name: V-set domain-containing T-cell activation inhibitor 1
• Protein Family: V-set domain containing T-cell activation inhibitor
• UniProt Gene Name: VTCN1
• UniProt Synonym Gene Names: B7H4
• UniProt Entry Name: VTCN1_HUMAN

NCBI Description

This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

VTCN1: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: receptor binding

Biological Process: response to protozoan; immune system process; positive regulation of T cell proliferation; negative regulation of T cell activation