Rabbit anti-Human DDX19B Polyclonal Antibody | anti-DDX19B antibody

DDX19B, NT (DDX19B, DBP5, DDX19, TDBP, ATP-dependent RNA helicase DDX19B, DEAD box RNA helicase DEAD5, DEAD box protein 19B) (FITC)

Cat. Num. AAA6291368

• Gene Names: DDX19B; DBP5; RNAh; DDX19
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DDX19B; Polyclonal Antibody; NT (DDX19B; DBP5; DDX19; TDBP; ATP-dependent RNA helicase DDX19B; DEAD box RNA helicase DEAD5; DEAD box protein 19B) (FITC); DEAD box protein 19B; anti-DDX19B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DDX19B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DDX19B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-36 amino acids from the N-terminal region of human DDX19B.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DDX19B Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DDX19B antibody detected the DDX19B protein (arrow).)

Related Product Information for anti-DDX19B antibody

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-DDX19B antibody

Antibodies; DEAD Box Proteins

References

Collins, R., et al. J. Biol. Chem. 284(16):10296-10300(2009). von Moeller, H., et al. Nat. Struct. Mol. Biol. 16(3):247-254(2009). Lamesch, P., et al. Genomics 89(3):307-315(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Yin, L., et al. Reprod. Fertil. Dev. 14 (3-4), 185-189 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 6005743
• NCBI GeneID: 11269
• NCBI Accession #: NP_009173.1; NP_001014451.1; NP_001014449.1; NP_060802.1
• NCBI GenBank Nucleotide #: NM_007242.5
• UniProt Accession #: Q9UMR2; Q6FIB7; Q6IAE0; Q96KE7; Q9H0U0; B3KNE9; B4DXS6; E7EMK4
• Molecular Weight: 51,063 Da
• NCBI Official Full Name: ATP-dependent RNA helicase DDX19B isoform 1
• NCBI Official Synonym Full Names: DEAD-box helicase 19B
• NCBI Official Symbol: DDX19B
• NCBI Official Synonym Symbols: DBP5; RNAh; DDX19
• NCBI Protein Information: ATP-dependent RNA helicase DDX19B
• UniProt Protein Name: ATP-dependent RNA helicase DDX19B
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DDX19B
• UniProt Synonym Gene Names: DBP5; DDX19; TDBP
• UniProt Entry Name: DD19B_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX19B: ATP-dependent RNA helicase involved in mRNA export from the nucleus. Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; EC 3.6.4.13; Helicase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nuclear membrane; membrane; cytoplasm; nuclear envelope; nuclear pore

Molecular Function: RNA binding; helicase activity; ATP binding

Biological Process: mRNA export from nucleus; protein transport