Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

ELISA (EIA) (ELISA analysis:|Coating: Plasma (diluted 1:1000) from 17beta-estradiol treated (E2) or control (C) fish. Purified vitellogenin (Vtg; 500ng/well).|Primary antibody: MBS602800)

Mouse Vitellogenin Monoclonal Antibody | anti-Vg antibody

Vitellogenin (killifish)

Gene Names
Vg; GB13999
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
Vitellogenin; Monoclonal Antibody; Vitellogenin (killifish); Anti -Vitellogenin (killifish); anti-Vg antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
6D836
Specificity
Recognizes vitellogenin from killifish (Fundulus heteroclitus) and white perch (Morone americanus). Cossreacts with vitellogenin from a variety of different species including common carp (Cyprinus carpio), wrasse (Ctenolabrus rupestris), tilapia (Oreochromis niloticus), halibut (Hippoglossus hippoglossus) and sheepshead minnow (Cyprinodon variegatus). Does not react with vitellogenin from bowfin (Amia calva) or with egg yolk from swordfish (Xiphias gladius).
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
Applicable Applications for anti-Vg antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:100-1:1000
Western Blot: 1:100-1:500
Immunogen
Vitellogenin from plasma of 17beta-estradiol treated killifish (Fundulus heteroclitus), white perch (Morone americanus), Bowfish (Amia Calva). Egg yolk from swordfish (Xiphias gladius).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 

ELISA (EIA)

(ELISA analysis:|Coating: Plasma (diluted 1:1000) from 17beta-estradiol treated (E2) or control (C) fish. Purified vitellogenin (Vtg; 500ng/well).|Primary antibody: MBS602800)

ELISA (EIA) (ELISA analysis:|Coating: Plasma (diluted 1:1000) from 17beta-estradiol treated (E2) or control (C) fish. Purified vitellogenin (Vtg; 500ng/well).|Primary antibody: MBS602800)

Western Blot (WB)

(Western Blot analysis:|Samples: Plasma (1:200, 15ul/well) from 17beta-estradiol treated (E2) or control (C) fish. Purified vitellogenin (Vtg; 1ug/well)|Primary antibody: MBS602800 diluted 1:100)

Western Blot (WB) (Western Blot analysis:|Samples: Plasma (1:200, 15ul/well) from 17beta-estradiol treated (E2) or control (C) fish. Purified vitellogenin (Vtg; 1ug/well)|Primary antibody: MBS602800 diluted 1:100)
Related Product Information for anti-Vg antibody
Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.
Product Categories/Family for anti-Vg antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
201,048 Da
NCBI Official Full Name
vitellogenin
NCBI Official Synonym Full Names
vitellogenin<
NCBI Official Symbol
Vg
NCBI Official Synonym Symbols
GB13999
NCBI Protein Information
vitellogenin
UniProt Protein Name
Vitellogenin
Protein Family
UniProt Gene Name
Vg
UniProt Entry Name
VIT_APIME

Uniprot Description

Function: Precursor of the egg-yolk proteins that are sources of nutrients during embryonic development

By similarity. Involved in the differentiation of honeybee larvae into queens. Ref.2

Subcellular location: Secreted.

Tissue specificity: Accumulates in the hemolymph. Represents up to 70% of the queen's hemolymph proteins. During the first week of the worker adult life, when it becomes a nurse bee and performs brood-rearing tasks, the vitellogenin titer increases and may account for up to 40% of the total hemolymph proteins. Ref.1

Developmental stage: In queens, first expressed in mid-late pupal stage and a high production is maintained throughout adult life. In workers, observed in late pupal stage and expressed at low levels in adults. Levels can increase in some young workers if an egg-laying queen is missing to the colony. Also detected in drones, but in freshly molted adults and not in pupae. Ref.1

Induction: By MRJP1 during the differentiation of honeybee larvae into queens. Ref.2

Sequence similarities: Contains 1 vitellogenin domain.Contains 1 VWFD domain.

Research Articles on Vg

Similar Products

Product Notes

The Vg vg (Catalog #AAA602800) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Vitellogenin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:100-1:1000 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the Vg vg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vitellogenin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.