Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of A4GNT expression in HEK293T (A), mouse testis (B), rat kidney (C) whole cell lysates.)

Rabbit A4GNT Polyclonal Antibody | anti-A4GNT antibody

Anti-A4GNT Antibody

Gene Names
A4GNT; alpha4GnT
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
A4GNT; Polyclonal Antibody; Anti-A4GNT Antibody; Alpha-1; 4-N-acetylglucosaminyltransferase; Alpha4GnT; anti-A4GNT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of A4GNT protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
340
Applicable Applications for anti-A4GNT antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human A4GNT. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of A4GNT expression in HEK293T (A), mouse testis (B), rat kidney (C) whole cell lysates.)

Western Blot (WB) (Western blot analysis of A4GNT expression in HEK293T (A), mouse testis (B), rat kidney (C) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of A4GNT staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of A4GNT staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-A4GNT antibody
Rabbit polyclonal antibody to A4GNT

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,497 Da
NCBI Official Full Name
alpha-1,4-N-acetylglucosaminyltransferase
NCBI Official Synonym Full Names
alpha-1,4-N-acetylglucosaminyltransferase
NCBI Official Symbol
A4GNT
NCBI Official Synonym Symbols
alpha4GnT
NCBI Protein Information
alpha-1,4-N-acetylglucosaminyltransferase
UniProt Protein Name
Alpha-1,4-N-acetylglucosaminyltransferase
UniProt Gene Name
A4GNT
UniProt Synonym Gene Names
Alpha4GnT
UniProt Entry Name
A4GCT_HUMAN

NCBI Description

This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane. [provided by RefSeq, Jul 2008]

Uniprot Description

A4GNT: Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O- glycans. Belongs to the glycosyltransferase 32 family.

Protein type: Membrane protein, integral; Transferase; EC 2.4.1.-

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: acetylglucosaminyltransferase activity

Biological Process: protein amino acid O-linked glycosylation; glycoprotein biosynthetic process; carbohydrate metabolic process; negative regulation of epithelial cell proliferation

Research Articles on A4GNT

Similar Products

Product Notes

The A4GNT a4gnt (Catalog #AAA822190) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-A4GNT Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's A4GNT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IH (1/100 - 1/200). Researchers should empirically determine the suitability of the A4GNT a4gnt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "A4GNT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.