VISTA antibody set

VISTA Detection Set (Risk Free)

Cat. Num. AAA154699

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Antibodies are supplied as protein A purified IgG1 and IgG2b
• Synonyms: VISTA; Monoclonal Antibody Set; VISTA Detection Set (Risk Free); VISTA antibody set

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, IgG2b
• Purity/Purification: Antibodies are supplied as protein A purified IgG1 and IgG2b
• Form/Format: Liquid. PBS containing 0.02% sodium azide.
• Concentration: Antibody 1mg/mL (varies by lot)

• Applicable Applications for VISTA antibody set: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)-Paraffin, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Immunogen: VISTA antibodies were raised against the extracellular domain of human VISTA.
• Conjugation: Unconjugated
• Preparation and Storage: Antibodies can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Related Product Information for VISTA antibody set

VISTA Antibody: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation. Risk-Free antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-Free means they are guaranteed to work for their approved applications.

References

Mayya V., et al. Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46.
Sakr M.A., et al.,GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. 2010, Cancer Sci. 101:2368-2374.