Mouse Visinin-like Protein 1 Monoclonal Antibody | anti-VSNL1 antibody

Visinin-like Protein 1 (VSNL1, VILIP, VISL1, VLP-1, Hippocalcin-like Protein 3, HLP3)

Cat. Num. AAA6011374

• Gene Names: VSNL1; HLP3; VILIP; HPCAL3; HUVISL1; VILIP-1
• Reactivity: Bovine, Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Visinin-like Protein 1; Monoclonal Antibody; Visinin-like Protein 1 (VSNL1; VILIP; VISL1; VLP-1; Hippocalcin-like Protein 3; HLP3); Anti -Visinin-like Protein 1 (VSNL1; anti-VSNL1 antibody

• Host: Mouse
• Reactivity: Bovine, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 13B870
• Specificity: Recognizes VSNL1 from human, bovine, mouse and rat. Since VSNL1 is highly conserved in primary sequence, it is likely that the antibody is effective on other species also.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 10mM sodium azide.

• Applicable Applications for anti-VSNL1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 1:500-1:1000; Western Blot: 1:1000-1:2000 using chemiluminescence.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot of bovine cerebellum homogenate stained with MBS6011374 in lane 10. Note the strong clean band running at 22kD. Lane 11 shows the same material stained with our alternate antibody to VSNL1, 031446, which binds to the same band.)

Testing Data

(Confocal image of adult rat cerebellar cortex stained with MBS6011374 (green), chicken polyclonal antibody to MAP2 031388 (red) and DNA (blue). The MBS6011374 antibody reveals synapses in the molecular layer (ML) strongly. Synaptic regions are also seen in the granule cell layer (GC). The perikarya of Purkinje cells (PC) are revealed with MAP2 antibody (4). Littel staining is seen in the white matter (WM).)

Related Product Information for anti-VSNL1 antibody

Visinin was originally isolated biochemically from chicken retina as a major protein of about 24kD on SDS-PAGE (1). Following cloning and sequencing of visinin, several visinin like proteins were discovered by homology screening (2, 3). One of these, Visinin-like protein 1 is a small Calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression (4, 5). It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein was discovered independently by several groups and is therefore also sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1. The protein belongs to the large superfamly of calmodulin and paravalbumin type proteins which function by binding Calcium ions. Calcium binding alters the confomation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like protein 1 has four "EF hand" domains, which are negatively charged helix-turn-helix peptides which are responsible for Calcium binding. Visinin-like protein 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of 22kD. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury (6, 7, 8). The HGNC name for this protein is VSNL1.

Product Categories/Family for anti-VSNL1 antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 21361559
• NCBI GeneID: 7447
• NCBI Accession #: NP_003376.2
• NCBI GenBank Nucleotide #: NM_003385.4
• UniProt Accession #: P62760; P28677; P29103; P42323; Q9UM20; D6W515
• Molecular Weight: 22,142 Da
• NCBI Official Full Name: visinin-like protein 1
• NCBI Official Synonym Full Names: visinin-like 1
• NCBI Official Symbol: VSNL1
• NCBI Official Synonym Symbols: HLP3; VILIP; HPCAL3; HUVISL1; VILIP-1
• NCBI Protein Information: visinin-like protein 1; VLP-1; hippocalcin-like protein 3
• UniProt Protein Name: Visinin-like protein 1
• Protein Family: Visinin-like protein
• UniProt Gene Name: VSNL1
• UniProt Synonym Gene Names: VISL1; VILIP; VLP-1; HLP3
• UniProt Entry Name: VISL1_HUMAN

NCBI Description

This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

VSNL1: Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner. Belongs to the recoverin family.

Chromosomal Location of Human Ortholog: 2p24.3

Molecular Function: calcium ion binding

Biological Process: calcium-mediated signaling