Mouse anti-Rat, Mouse VGAT Monoclonal Antibody | anti-VGAT antibody
VGAT Antibody, Clone D9: ATTO 488
Gene Names
Slc32a1; VGAT; Viaat; R75019
Reactivity
Rat, Mouse
Applications
Western Blot, ELISA
Purity
Protein A Purified
Synonyms
VGAT; Monoclonal Antibody; VGAT Antibody; Clone D9: ATTO 488; Mouse Anti-Mouse VGAT Monoclonal IgG2a; GABA and glycine transporter antibody; hVIAAT antibody; SLC32A 1 antibody; Slc32a1 antibody; solute carrier family 32 (GABA vesicular transporter) member 1 antibody; Solute carrier family 32 member 1 antibody; Vesicular GABA Amino Acid Transporter antibody; Vesicular GABA transporter antibody; Vesicular inhibitory amino acid transporter antibody; VGAT antibody; VIAAT antibody; VIAAT_HUMAN antibody; anti-VGAT antibody
Host
Mouse
Reactivity
Rat, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
D9
Specificity
Detects 57 kDa.
Purity/Purification
Protein A Purified
Form/Format
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration
1mg/ml (varies by lot)
Sequence Length
525
Applicable Applications for anti-VGAT antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500
Immunogen
Synthetic peptide from the N-terminal region of Mouse VGAT protein
Immunogen Species
Mouse
Tissue Specificity
Retina. Expressed throughout the horizontal cells and/or terminals.
Conjugate
ATTO 488
Cellular Localization
Cytoplasmic vesicle membrane
Certificate of Analysis
A 1:500 dilution was sufficient for detection of VGAT in 15 ug of Rat Brain Membrane by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Preparation and Storage
Store at -20 degree C
Shipping Temperature: Blue Ice or 4 degree C
Shipping Temperature: Blue Ice or 4 degree C
Related Product Information for anti-VGAT antibody
Background: Vesicular GABA Transporter (VGAT) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporters. VGAT functions in the uptake of GABA and glycine into synaptic vesicles. Since the protein is bound to synaptic vesicles, VGAT antibodies are excellent markers of presynaptic GABAergic and glycinergic neurons.
Product Categories/Family for anti-VGAT antibody
References
1. McIntire S.L., et al. (1997) Nature. 389(6653): 870-876.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
vesicular inhibitory amino acid transporter
NCBI Official Synonym Full Names
solute carrier family 32 (GABA vesicular transporter), member 1
NCBI Official Symbol
Slc32a1
NCBI Official Synonym Symbols
VGAT; Viaat; R75019
NCBI Protein Information
vesicular inhibitory amino acid transporter
UniProt Protein Name
Vesicular inhibitory amino acid transporter
UniProt Gene Name
Slc32a1
UniProt Synonym Gene Names
Vgat; Viaat; mVGAT; mVIAAT
UniProt Entry Name
VIAAT_MOUSE
Uniprot Description
SLC32A1: Involved in the uptake of GABA and glycine into the synaptic vesicles. Belongs to the amino acid/polyamine transporter 2 family.
Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter
Cellular Component: synaptic vesicle; neuron projection; membrane; integral to plasma membrane; dendrite; presynaptic active zone; integral to membrane; intracellular organelle; synapse; cytoplasmic vesicle
Molecular Function: glycine transmembrane transporter activity; gamma-aminobutyric acid:hydrogen symporter activity
Biological Process: glycine transport; proton transport; transport; neurotransmitter transport; gamma-aminobutyric acid transport
Research Articles on VGAT
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Product Notes
The VGAT slc32a1 (Catalog #AAA8005783) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The VGAT Antibody, Clone D9: ATTO 488 reacts with Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VGAT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500. Researchers should empirically determine the suitability of the VGAT slc32a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VGAT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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