Flow Cytometry (FC/FACS)
(Fig.1: GM-1007. BOSC23 cells were transiently transfected with an expression vector encoding either human VEGFR2 (green curve), pig VEGFR2 (blue curve) or an irrelevant protein (control transfectant, red curve). Binding of BEP-5D8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with human and pig VEGFR2 transfected cells.)
Rat VEGFR2 Monoclonal Antibody | anti-VEGFR2 antibody
Rat anti-human VEGFR2 monoclonal antibody
ELISA: 1:200 - 1:400
C-ELISA: 1:200 - 1:400
Long term: -20 degree C (avoid repeated freezing and thawing)
Flow Cytometry (FC/FACS)
(Fig.1: GM-1007. BOSC23 cells were transiently transfected with an expression vector encoding either human VEGFR2 (green curve), pig VEGFR2 (blue curve) or an irrelevant protein (control transfectant, red curve). Binding of BEP-5D8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with human and pig VEGFR2 transfected cells.)
Flow Cytometry (FC/FACS)
(Fig.1: GM-1007. BOSC23 cells were transiently transfected with an expression vector encoding either human VEGFR2 (green curve), pig VEGFR2 (blue curve) or an irrelevant protein (control transfectant, red curve). Binding of BEP-5D8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with human and pig VEGFR2 transfected cells.)
Testing Data
(Fig.2: SDS-PAGE analysis of purified BEP-5D8 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified BEP-5D8 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent 3.)
Testing Data
(Fig.2: SDS-PAGE analysis of purified BEP-5D8 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified BEP-5D8 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent 3.)
2. Youssoufian H., Hicklin D.J., Rowinsky E.K. (2007) Monoclonal antibodies to the vascular endothelial growth factor receptor-2 in cancer therapy. Clin. Cancer Res. 13 (18 Suppl): 5544- 5528.
NCBI and Uniprot Product Information
NCBI Description
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]
Uniprot Description
VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability.
Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; VEGFR family
Chromosomal Location of Human Ortholog: 4q11-q12
Cellular Component: cell junction; early endosome; endoplasmic reticulum; endosome; Golgi apparatus; integral to plasma membrane; lipid raft; plasma membrane
Molecular Function: growth factor binding; Hsp90 protein binding; integrin binding; protein binding; protein-tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor receptor activity
Biological Process: angiogenesis; cell migration during sprouting angiogenesis; embryonic hemopoiesis; extracellular matrix organization and biogenesis; negative regulation of apoptosis; peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of focal adhesion formation; positive regulation of macroautophagy; positive regulation of MAPKKK cascade; positive regulation of mitochondrial depolarization; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of positive chemotaxis; positive regulation of protein amino acid phosphorylation; protein amino acid autophosphorylation; regulation of cell shape; transmembrane receptor protein tyrosine kinase signaling pathway; vascular endothelial growth factor receptor signaling pathway; vasculogenesis
Disease: Hemangioma, Capillary Infantile
Research Articles on VEGFR2
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Product Notes
The VEGFR2 kdr (Catalog #AAA468053) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's VEGFR2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), C-ELISA (EIA), ELISA (EIA). Flow Cytometry: 1.2 mug/10^6 cells ELISA: 1:200 - 1:400 C-ELISA: 1:200 - 1:400. Researchers should empirically determine the suitability of the VEGFR2 kdr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VEGFR2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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