Mouse VCAM1 Monoclonal Antibody | anti-VCAM1 antibody

Mouse monoclonal VCAM1 antibody

Cat. Num. AAA5307687

• Gene Names: VCAM1; CD106; INCAM-100
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: VCAM1; Monoclonal Antibody; Mouse monoclonal VCAM1 antibody; VCAM1 antibody; anti-VCAM1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5B12
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5-1 mg/ml (varies by lot)
• Sequence Length: 739

• Applicable Applications for anti-VCAM1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:150; WB: 1:500-2000
• Immunogen: VCAM1 antibody was raised in Mouse using full length human recombinant protein of human VCAM1 produced in HEK293T cell as the immunogen.
• Cross-Reactivity: Human
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of VCAM1 protein in paraffin embedded Human breast tissue using VCAM1 antibody)

Product Categories/Family for anti-VCAM1 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 340194
• NCBI GeneID: 7412
• Molecular Weight: 74,346 Da
• NCBI Official Full Name: vascular cell adhesion molecule-1
• NCBI Official Synonym Full Names: vascular cell adhesion molecule 1
• NCBI Official Symbol: VCAM1
• NCBI Official Synonym Symbols: CD106; INCAM-100
• NCBI Protein Information: vascular cell adhesion protein 1
• UniProt Protein Name: Vascular cell adhesion protein 1
• Protein Family: Vascular cell adhesion protein
• UniProt Gene Name: VCAM1
• UniProt Synonym Gene Names: V-CAM 1; VCAM-1

NCBI Description

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p21.2

Cellular Component: apical part of cell; cell surface; early endosome; endoplasmic reticulum; external side of plasma membrane; extracellular space; filopodium; Golgi apparatus; microvillus; plasma membrane; podosome

Molecular Function: amine oxidase activity; cell adhesion molecule binding; integrin binding

Biological Process: amine metabolic process; B cell differentiation; cell adhesion; cell-matrix adhesion; extracellular matrix organization and biogenesis; heterophilic cell adhesion; leukocyte adhesion; leukocyte tethering or rolling; membrane to membrane docking; positive regulation of T cell proliferation; regulation of immune response