Western Blot (WB)
(WB: CTLL cell lysate probed with V2118 (0.5ug/ml) .)
Mouse anti-Human, Mouse Vasodilator Stimulated Phosphoprotein Monoclonal Antibody | anti-VASP antibody
Vasodilator Stimulated Phosphoprotein, phosphorylated (Ser239) (VASP) (Biotin)
Dilution: ELISA: 0.05ug/ml, as reported by an independent laboratory.
Western Blot: 0.5-2ug/ml detected phosphorylated VASP in RIPA lysates from CTLL cells. CTLL cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with V2118 (0.5ug/ml). Proteins were visualized using a Gt xMo IgG (HRP)* and a chemiluminescence detection system.
Immunocytochemistry: An independent laboratory has reported that 3ug/ml antibody showed positive immunostaining in SNP or 8pCPT-cGMP-treated human platelets, xed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.
Flow Cytometry: 2.4ug/ml using xed and permeabilized human platelets, as reported by an independent laboratory.
Immunoprecipitation: Immunoprecipitates phosphorylated VASP from vanadate-treated A431 cells, as reported by an independent laboratory.
Western Blot (WB)
(WB: CTLL cell lysate probed with V2118 (0.5ug/ml) .)
Western Blot (WB)
(WB: CTLL cell lysate probed with V2118 (0.5ug/ml) .)
NCBI and Uniprot Product Information
NCBI Description
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq]
Uniprot Description
VASP: vasodilator-stimulated phosphoprotein. Actin- and profilin-binding microfilament-associated protein. The phosphorylation of VASP is dynamically regulated by cellular adhesion to extracellular matrix.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 19q13.32
Cellular Component: filopodium membrane; focal adhesion; tight junction; cytoplasm; plasma membrane; cytosol; actin cytoskeleton
Molecular Function: protein binding; actin binding; SH3 domain binding; profilin binding
Biological Process: axon guidance; positive regulation of actin filament polymerization; actin polymerization and/or depolymerization; neural tube closure; T cell receptor signaling pathway; protein homotetramerization
Research Articles on VASP
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Product Notes
The VASP vasp (Catalog #AAA603306) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Vasodilator Stimulated Phosphoprotein, phosphorylated (Ser239) (VASP) (Biotin) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Vasodilator Stimulated Phosphoprotein can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Suitable for use in ELISA, Western Blot, Immunocytochemistry, Flow Cytometry and Immunoprecipitation. Dilution: ELISA: 0.05ug/ml, as reported by an independent laboratory. Western Blot: 0.5-2ug/ml detected phosphorylated VASP in RIPA lysates from CTLL cells. CTLL cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with V2118 (0.5ug/ml). Proteins were visualized using a Gt xMo IgG (HRP)* and a chemiluminescence detection system. Immunocytochemistry: An independent laboratory has reported that 3ug/ml antibody showed positive immunostaining in SNP or 8pCPT-cGMP-treated human platelets, xed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X. Flow Cytometry: 2.4ug/ml using xed and permeabilized human platelets, as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates phosphorylated VASP from vanadate-treated A431 cells, as reported by an independent laboratory. Researchers should empirically determine the suitability of the VASP vasp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vasodilator Stimulated Phosphoprotein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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