Mouse anti-Human Vascular Endothelial Growth Factor 121 (VEGF121) Monoclonal Antibody | anti-VEGF121 antibody

Monoclonal Antibody to Vascular Endothelial Growth Factor 121 (VEGF121)

Cat. Num. AAA2090565

• Gene Names: VEGFA; VPF; VEGF; MVCD1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Protein A + Protein G affinity chromatography
• Synonyms: Vascular Endothelial Growth Factor 121 (VEGF121); Monoclonal Antibody; Monoclonal Antibody to Vascular Endothelial Growth Factor 121 (VEGF121); anti-VEGF121 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b Kappa
• Clone Number: C2
• Specificity: The antibody is a mouse monoclonal antibody raised against VEGF121. It has been selected for its ability to recognize VEGF121 in immunohistochemical staining and western blotting.
• Purity/Purification: Protein A + Protein G affinity chromatography
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 412

• Applicable Applications for anti-VEGF121 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: Western Blot: 0.5-5ug/mL; Immunocytochemistry in formalin fixed cells: 5-30ug/mL; Immunohistochemistry in formalin fixed frozen section: 5-30ug/mL; Immunohistochemistry in paraffin section: 5-30ug/mL
• Immunogen: Recombinant Vascular Endothelial Growth Factor 121 (VEGF121)
• Cross Reactivity: Human
• Buffer Formulation: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2059841
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant VEGF121, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Thyroid Cancer Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Lung Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 76781480
• NCBI GeneID: 7422
• NCBI Accession #: NP_001020537.2
• NCBI GenBank Nucleotide #: NM_001025366.2
• UniProt Accession #: P15692; O60720; O75875; Q074Z4; Q16889; B5BU86; H0Y2S8; H0Y407; H0Y414; H0Y462; H0Y8N2; H3BLW7
• Molecular Weight: 34,406 Da
• NCBI Official Full Name: vascular endothelial growth factor A isoform a
• NCBI Official Synonym Full Names: vascular endothelial growth factor A
• NCBI Official Symbol: VEGFA
• NCBI Official Synonym Symbols: VPF; VEGF; MVCD1
• NCBI Protein Information: vascular endothelial growth factor A
• UniProt Protein Name: Vascular endothelial growth factor A
• UniProt Gene Name: VEGFA
• UniProt Synonym Gene Names: VEGF; VEGF-A; VPF

NCBI Description

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]

Uniprot Description

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development ().