Rat anti-Mouse VAP-1 Monoclonal Antibody | anti-VAP-1 antibody
VAP-1, Mouse, mAb 7-88
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation
Synonyms
VAP-1; Monoclonal Antibody; Mouse; mAb 7-88; Amine oxidase copper containing 3; AOC3; SSAO;- membrane copper amine oxidase; anti-VAP-1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2b
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Sequence Length
20
Applicable Applications for anti-VAP-1 antibody
F, Flow Cytometry (FC/FACS), FS, Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
Application Notes: FC: Antibody 7-88 stains the extracellular domain of mouse VAP-1 in CHO cells transfected with mouse VAP-1 cDNA.. As positive control- anti-VAP-1 clone 7-106 was used and as negative control an isotype-matched control antibody. (Ref. 2)
Application Use: For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, dilutions have to be optimized in user’s experimental setting.
Application Use: For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, dilutions have to be optimized in user’s experimental setting.
Immunogen
Vessels from mouse lymph nodes
Positive Control
Mouse VAP-1-transfected CHO cells (ref. 2)
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Related Product Information for anti-VAP-1 antibody
Product Description: The monoclonal antibody 7-88 recognizes mouse Vascular Adhesion Protein-1 (VAP-1) which- is a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. It contains a short N-terminal cytoplasmic tail, a single membrane-spanning domain and a large extracellular part. A soluble form of VAP-1 (sVAP-1) has been described, which presumably results from the proteolytic cleavage of membrane-bound VAP-1. Structurally VAP-1 belongs to enzymes called semicarbamizide-sensitive amine oxidases, which contain copper as a cofactor. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia.
VAP-1 is expressed in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. In endothelial cells the majority of VAP-1 is stored within intracellular granules and translocated to the surface upon inflammation where it regulates leukocyte tissue infiltration. Furthermore, the end-products formed by VAP-1 can also regulate leukocyte migration by signaling effects, have insulin-like effects in energy metabolism, and can cause vascular damage by direct cytotoxicity.
In- white adipose tissue of obese and diabetic db-/- mice increased expression of VAP-1 has been observed suggesting that it contributes- to the arthrosclerosis and vascular dysfunction observed in these diseases. Moreover, inhibition of VAP-1reduced the accumulation of myeloid cells into tumors and attenuates tumor growth.
The monoclonal antibody 7-88 inhibits migration of granulocytes and monocytes in acute models of inflammation.
VAP-1 is expressed in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. In endothelial cells the majority of VAP-1 is stored within intracellular granules and translocated to the surface upon inflammation where it regulates leukocyte tissue infiltration. Furthermore, the end-products formed by VAP-1 can also regulate leukocyte migration by signaling effects, have insulin-like effects in energy metabolism, and can cause vascular damage by direct cytotoxicity.
In- white adipose tissue of obese and diabetic db-/- mice increased expression of VAP-1 has been observed suggesting that it contributes- to the arthrosclerosis and vascular dysfunction observed in these diseases. Moreover, inhibition of VAP-1reduced the accumulation of myeloid cells into tumors and attenuates tumor growth.
The monoclonal antibody 7-88 inhibits migration of granulocytes and monocytes in acute models of inflammation.
References
1. Abella, A et al; Adipocytes release a soluble form of VAP-1/SSAO by a metalloproteasedependent process and in a regulated manner. Diabetologia 2004, 47: 429
2. Merinen, M et al; Vascular adhesion protein-1 is involved in both acute and chronic inflammation in the mouse. Am J Path 2005, 166: 793
3. Bonder, C et al; Rules of recruitment for Th1 and Th2 lymphocytes in inflamed liver: a role for alpha-4 integrin and vascular adhesion protein-1. Immunity 2005, 23: 153
4. Marttila-Ichihara, F et al; Small-molecule inhibitors of vascular adhesion protein-1 reduce the accumulation of myeloid cells into tumors and attenuate tumor growth in mice. J Immunol 2010, 184: 3164
5. Iffi degree -Solt©sz, Z et al; Increased primary amine oxidase expression and activity in white adipose tissue of obese and diabetic db-/- mice. J Neural Transm 2011, DOI: 10.1007/s00702-011- 0586-9
2. Merinen, M et al; Vascular adhesion protein-1 is involved in both acute and chronic inflammation in the mouse. Am J Path 2005, 166: 793
3. Bonder, C et al; Rules of recruitment for Th1 and Th2 lymphocytes in inflamed liver: a role for alpha-4 integrin and vascular adhesion protein-1. Immunity 2005, 23: 153
4. Marttila-Ichihara, F et al; Small-molecule inhibitors of vascular adhesion protein-1 reduce the accumulation of myeloid cells into tumors and attenuate tumor growth in mice. J Immunol 2010, 184: 3164
5. Iffi degree -Solt©sz, Z et al; Increased primary amine oxidase expression and activity in white adipose tissue of obese and diabetic db-/- mice. J Neural Transm 2011, DOI: 10.1007/s00702-011- 0586-9
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
vascular adhesion protein-1, VAP-1=90 kda lymphocyte binding endothelial cell adhesion molecule {N-terminal}, partial
Protein Family
Similar Products
Product Notes
The VAP-1 (Catalog #AAA584382) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The VAP-1, Mouse, mAb 7-88 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VAP-1 can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), FS, Immunofluorescence (IF), Immunoprecipitation (IP). Application Notes: FC: Antibody 7-88 stains the extracellular domain of mouse VAP-1 in CHO cells transfected with mouse VAP-1 cDNA. As positive control- anti-VAP-1 clone 7-106 was used and as negative control an isotype-matched control antibody. (Ref. 2) Application Use: For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, dilutions have to be optimized in user’s experimental setting. Researchers should empirically determine the suitability of the VAP-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VAP-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
