Mouse V5-TAG Monoclonal Antibody | anti-V5-TAG antibody
MOUSE ANTI V5-TAG
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, immunoassay, Radioimmunoassay, Western Blot
Synonyms
V5-TAG; Monoclonal Antibody; MOUSE ANTI V5-TAG; PK-TAG; anti-V5-TAG antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
SV5-Pk1
Specificity
Mouse anti V5-Tag, clone SV5-Pk1 recognizes the sequence, IPNPLLGLD, present on the P/V proteins of the paramyxovirus, SV5 (Dunn et al.1999). Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labeled with tags containing this sequence (Randall et al.1993 and Zhao et al. 2005).
Form/Format
Purified IgG - liquid
Concentration
IgG concentration: 1 mg/ml (varies by lot)
Applicable Applications for anti-V5-TAG antibody
ELISA, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry - Frozen (IHC-F), Immunoprecipitation (IP), Radioimmunoassays (RIA), Western Blotting (WB)
Application Notes
Suggested Dilutions:
ELISA: 1/1000 - 1/5000
Western Blotting: 1/1000 - 1/5000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA: 1/1000 - 1/5000
Western Blotting: 1/1000 - 1/5000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Target Species
Viral
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Paramyxovirus Simian-Virus 5 (SV5)
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt.
When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
V protein
NCBI Official Symbol
V/P
NCBI Protein Information
phosphoprotein; V protein
Research Articles on V5-TAG
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Product Notes
The V5-TAG (Catalog #AAA212110) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's V5-TAG can be used in a range of immunoassay formats including, but not limited to, ELISA, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry - Frozen (IHC-F), Immunoprecipitation (IP), Radioimmunoassays (RIA), Western Blotting (WB). Suggested Dilutions: ELISA: 1/1000 - 1/5000 Western Blotting: 1/1000 - 1/5000 Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the V5-TAG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "V5-TAG, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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