Rabbit ULK1 Monoclonal Antibody | anti-Ulk1 antibody

ULK1, phosphorylated (Ser555) (Serine/Threonine-protein Kinase ULK1, Unc-51-like Kinase 1, KIAA0722)

Cat. Num. AAA644095

• Gene Names: Ulk1; Unc51.1; AU041434; mKIAA0722
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Supernatant; Supernatant
• Synonyms: ULK1; Monoclonal Antibody; phosphorylated (Ser555) (Serine/Threonine-protein Kinase ULK1; Unc-51-like Kinase 1; KIAA0722); Anti -ULK1; anti-Ulk1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: D1H4
• Specificity: Recognizes endogenous levels of human ULK1, phosphorylated at Ser555. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Ulk1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:100
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser555 of human ULK1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of extracts from MCF7 cells, untreated or treated with oligomycin (0.5uM, 30 minutes), and C2C12 cells, untreated or treated with hydrogen peroxide (10 mM, 5 minutes), using MBS644095.)

Western Blot (WB)

(Western Blot analysis of extracts from MCF7 cells, untreated or treated with oligomycin (0.5uM, 30 minutes), using MBS644095 (left). Phoshpo-specificty is demonstrated by pre-incubating the antibody with phosphorylated (middle) or non-phoshporylated peptides (right) against a region surrounding Ser555 of ULK1.)

Related Product Information for anti-Ulk1 antibody

Two related serine/threonine kinases, UNC- 51-like kinase -1 and -2 (ULK1, ULK2), were discovered as mammalian homologs of the C. elegans gene UNC-51 in which mutants exhibited abnormal axonal extension and growth (1-4). Both proteins are widely expressed and contain an amino terminal kinase domain followed by a central proline/serine rich domain and a highly conserved carboxy-terminal domain (CTD). The roles of ULK1 and ULK2 in axon growth have been linked to studies showing that the kinases are localized to neuronal growth cones and are involved in endocytosis of critical growth factors such as NGF (5). Yeast two-hybrid studies found ULK1/2 associated with modulators of the endocytic pathway, SynGap, and syntenin (6). Structural similarity of ULK1/2 has also been recognized with the yeast autophagy protein Atg1/Apg1 (7). Knockdown experiments using siRNA demonstrated that ULK1 is essential for autophagy (8), a catabolic process for the degradation of bulk cytoplasmic contents (9,10). It appears that Atg1/ULK1 can act as a convergence point for multiple signals that control autophagy (11), and can bind to several autophagy-related (Atg) proteins, regulating phosphorylation states and protein trafficking (12-16). Phosphorylation of ULK1 by AMPK at Ser555 is critical for starvation-induced autophagy, cell survival under conditions of low nutrients and energy, and mitochondiral homeostasis (17).

Product Categories/Family for anti-Ulk1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 3136154
• NCBI GeneID: 22241
• UniProt Accession #: O70405; Q6PGB2
• Molecular Weight: 112,463 Da
• NCBI Official Full Name: UNC-51-like kinase ULK1
• NCBI Official Synonym Full Names: unc-51 like kinase 1
• NCBI Official Symbol: Ulk1
• NCBI Official Synonym Symbols: Unc51.1; AU041434; mKIAA0722
• NCBI Protein Information: serine/threonine-protein kinase ULK1; unc-51-like kinase 1; serine/threonine-protein kinase Unc51.1
• UniProt Protein Name: Serine/threonine-protein kinase ULK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Ulk1
• UniProt Entry Name: ULK1_MOUSE

Uniprot Description

ULK1: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. Interacts with GABARAP and GABARAPL2. Interacts (via C- terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation. Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Autophagy; Kinase, protein; Protein kinase, Other; Other group; ULK family

Cellular Component: neuron projection; cytoplasmic vesicle membrane; cell soma; membrane; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; cytosol

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; protein complex binding; Hsp90 protein binding; kinase activity; protein kinase binding; Rab GTPase binding; ATP binding; protein kinase activity

Biological Process: axon extension; protein amino acid autophosphorylation; neurite regeneration; negative regulation of collateral sprouting; signal transduction; protein amino acid phosphorylation; cerebellar granule cell differentiation; radial glia guided migration of granule cell; response to starvation; protein localization; peptidyl-serine phosphorylation; cellular response to nutrient levels; axonogenesis; regulation of gene expression; receptor internalization; Ras protein signal transduction; autophagy; regulation of autophagy; regulation of nerve growth factor receptor signaling pathway; phosphorylation; positive regulation of macroautophagy; positive regulation of autophagy; neurite development; autophagic vacuole formation