Mouse Ubiquitin Conjugating Enzyme E2S Monoclonal Antibody | anti-UBE2S antibody

Mouse Anti Human Ubiquitin Conjugating Enzyme E2S

Cat. Num. AAA140333

• Gene Names: UBE2S; EPF5; E2EPF; E2-EPF
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: UBE2S antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Ubiquitin Conjugating Enzyme E2S; Monoclonal Antibody; Mouse Anti Human Ubiquitin Conjugating Enzyme E2S; UBE2S Antibody; Mouse Anti Human; Ubiquitin-conjugating enzyme E2 S; Ubiquitin-protein ligase S; Ubiquitin carrier protein S; Ubiquitin-conjugating enzyme E2-24 kDa; E2-EPF5; E2-EPF; UBE2S; E2EPF; EPF5; anti-UBE2S antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a heavy chain and kappa light chain
• Clone Number: PAT2N6AT
• Purity/Purification: UBE2S antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 222

• Applicable Applications for anti-UBE2S antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: UBE2S antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250.
• Immunogen: Anti-human UBE2S mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human UBE2S amino acids 1-222 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-UBE2S antibody

Introduction: Ubiquitin-conjugating enzyme E2S (UBE2S) belongs to the ubiquitin-conjugating enzyme family. UBE2S is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a typical property of ubiquitin carrier proteins. UBE2S catalyzes the covalent attachment of ubiquitin to other proteins. UBE2S acts as a crucial factor of the anaphase promoting complex/cyclosome (APC/C), which is a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. UBE2S acts by purposely elongating ''Lys-11''-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, augmenting the degradation of APC/C substrates by the proteasome and promoting mitotic exit. UBE2S also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is nevertheless uncertain whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. UBE2S is also involved in ubiquitination and consequent degradation of VHL, resu

Product Categories/Family for anti-UBE2S antibody

Monoclonal Antibodies; Anti-Human Enzymes; Monoclonal Antibodies; Anti Human Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 112382377
• NCBI GeneID: 27338
• NCBI Accession #: NP_055316.2
• NCBI GenBank Nucleotide #: NM_014501.2
• Molecular Weight: 23,845 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 S
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2S
• NCBI Official Symbol: UBE2S
• NCBI Official Synonym Symbols: EPF5; E2EPF; E2-EPF
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 S; E2-EPF5; ubiquitin carrier protein S; ubiquitin-conjugating enzyme E2-24 kD; ubiquitin-conjugating enzyme E2-24 kDa; ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-protein ligase S
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 S
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2S
• UniProt Synonym Gene Names: E2EPF
• UniProt Entry Name: UBE2S_HUMAN

NCBI Description

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE2S: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination. Component of the APC/C complex. Interacts with CDC20, FZR1/CDH1 and VHL. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: EC 6.3.2.19; Ubiquitin ligase; Ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19q13.43

Cellular Component: anaphase-promoting complex; cytoplasm

Molecular Function: small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: anaphase-promoting complex activation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; protein modification process; exit from mitosis