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Immunohistochemistry (IHC) (Fig.1. Immunohistochemical analysis of paraffin-embedded Human Breast CarcinomaTissue using Ubiquitin Mouse mAb diluted at 1:200.)

Mouse Ubiquitin Monoclonal Antibody | anti-UBB antibody

Ubiquitin Mouse Monoclonal Antibody (6G6)

Gene Names
UBB; HEL-S-50
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Synonyms
Ubiquitin; Monoclonal Antibody; Ubiquitin Mouse Monoclonal Antibody (6G6); Mouse Anti-Ubiquitin Mouse Monoclonal Antibody (6G6); FLJ25987; MGC8385; Polyubiquitin B; RPS 27A; RPS27A; UBA 52; UBA 80; UBA52; UBA80; UBB_HUMAN; UBC; UBCEP 1; UBCEP 2; UBCEP1; UBCEP2; Ubiquitin B; anti-UBB antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Specificity
Ubiquitin protein detects endogenous levels of Ubiquitin.
Purity/Purification
Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form/Format
Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
128
Applicable Applications for anti-UBB antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
WB: 1:1000-1:2000
IHC-P: 1:100-1:200
Immunogen
Synthetic Peptide of Ubiquitin
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Fig.1. Immunohistochemical analysis of paraffin-embedded Human Breast CarcinomaTissue using Ubiquitin Mouse mAb diluted at 1:200.)

Immunohistochemistry (IHC) (Fig.1. Immunohistochemical analysis of paraffin-embedded Human Breast CarcinomaTissue using Ubiquitin Mouse mAb diluted at 1:200.)

Western Blot (WB)

(Fig.2. Western blot analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using Ubiquitin Mouse mAb diluted at 1:1000.)

Western Blot (WB) (Fig.2. Western blot analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using Ubiquitin Mouse mAb diluted at 1:1000.)
Related Product Information for anti-UBB antibody
UBB (ubiquitin B) encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBB consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of UBB are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Official Full Name
ubiquitin
NCBI Official Synonym Full Names
ubiquitin B
NCBI Official Symbol
UBB
NCBI Official Synonym Symbols
HEL-S-50
NCBI Protein Information
polyubiquitin-B
Protein Family

NCBI Description

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Research Articles on UBB

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Product Notes

The UBB (Catalog #AAA9700333) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Ubiquitin Mouse Monoclonal Antibody (6G6) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ubiquitin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: 1:1000-1:2000 IHC-P: 1:100-1:200. Researchers should empirically determine the suitability of the UBB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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