Rabbit Ubiquitin Monoclonal Antibody | anti-UBC antibody

Ubiquitin Antibody, Clone RGL3R: ATTO 390

Cat. Num. AAA809241

• Gene Names: UBB; HEL-S-50
• Reactivity: Species Independent
• Applications: Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: Ubiquitin; Monoclonal Antibody; Ubiquitin Antibody; Clone RGL3R: ATTO 390; Rabbit Anti-Human Ubiquitin Recombinant Monoclonal IgG; Polyubiquitin B Antibody; RPS27A Antibody; UBA52 Antibody; UBB Antibody; UBC Antibody; ubiquitin B Antibody; anti-UBC antibody

• Host: Rabbit
• Reactivity: Species Independent
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: RGL3R
• Specificity: Detects ubiquitined proteins, including poly-ubiquitinated proteins (K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins). Does not detect free ubiquitin.
• Purity/Purification: Protein A Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 229

• Applicable Applications for anti-UBC antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000; ELISA: 1:1000
• Target: Ubiquitin
• Immunogen: Ubiquitin conjugated lysozyme
• Immunogen Species: Human
• Cellular Localization: Cytoplasm; Nucleus
• Conjugate: ATTO 390
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western Blot analysis of Purified poly-ubiquitin chains showing detection of Multiple Ubiquitin protein using Rabbit Anti-Ubiquitin Monoclonal Antibody, Clone RGL3R. Lane 1: Molecular Weight Ladder (MW). Lane 2: K63 Poly Ubiquitin. Lane 3: K48 Poly Ubiquitin. Load: 2 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG:HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)

Related Product Information for anti-UBC antibody

Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(2, 3). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (4). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IkappaBalpha and p27 (5).

Product Categories/Family for anti-UBC antibody

Antibodies; Recombinant Monoclonal; Cell Signaling; Post-translational Modifications; Ubiquitination; Neuroscience; Neurodegeneration; Alzheimer's Disease

References

1. Wilkinson K.D. (1995) Annu. Rev. Nutr. 15:161-189. 2. Bonifacino J.S., et al. (1998) Annu Rev Cell Dev Biol. 14: 19-57. 3. Boston Biochem: "Ubiquitin Proteasome Pathway Overview" http://www.bostonbiochem.com/upp.php 4. Yang M., et al. (1998) J Exp Med. 187: 1835-1846. 5. Chen Z.J., et al. (1996) Cell 84: 853-862.

NCBI and Uniprot Product Information

• NCBI GI #: 528524471
• NCBI GeneID: 7314
• NCBI Accession #: NP_001268645.1
• NCBI GenBank Nucleotide #: NM_001281716.1
• UniProt Accession #: P0CG47; P02248; P02249; P02250; P62988; Q29120; Q6LBL4; Q6LDU5; Q8WYN8; Q91887; Q91888; Q9BWD6
• Molecular Weight: 25,762 Da
• NCBI Official Full Name: polyubiquitin-B
• NCBI Official Synonym Full Names: ubiquitin B
• NCBI Official Symbol: UBB
• NCBI Official Synonym Symbols: HEL-S-50
• NCBI Protein Information: polyubiquitin-B
• UniProt Protein Name: Polyubiquitin-B
• Protein Family: Ubiquitin
• UniProt Gene Name: UBB

NCBI Description

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.