Ubiquitin Monoclonal Antibody | anti-UBC antibody

Ubiquitin, monoclonal antibody, mouse

Cat. Num. AAA555677

• Gene Names: UBC; HMG20
• Reactivity: Bovine, Chicken, Human, Mouse, Porcine, Rat
• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity Purified
• Synonyms: Ubiquitin; Monoclonal Antibody; monoclonal antibody; mouse; anti-UBC antibody

• Reactivity: Bovine, Chicken, Human, Mouse, Porcine, Rat
• Clonality: Monoclonal
• Purity/Purification: Affinity Purified
• Form/Format: Liquid
• Sequence Length: 685

• Applicable Applications for anti-UBC antibody: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Testing Data

(Image: staining of cerebral cortex of an Alzheimer patient. Neurofibrillary tangles and dystrophic neurites associated with senile plaques stain strongly with this antibody. In the center is a typical neurofibrillary tangle containing neuron)

Testing Data

(Image: Blots of mono and polyubiquitin (lane 1), monoubiquitin only (2), and 100 mug total wet weight of cerebellum, cortex and brain stem respectively (lane 3-5). Material was run out on 20% SDS-PAGE and transferred electrophoretically to PVDF)

Related Product Information for anti-UBC antibody

Ubiquitin is a highly conserved globular 76 amino acid protein of about 8.5 kDa molecular weight. It has an important role in the targeting of proteins for proteolytic degradation. Proteins to be degraded are covalently coupled to the C-terminus of ubiquitin by means of ubiquitin ligases. The ubiquitin itself is frequently also ubiquitinated, producing a polyubiquitin chain. The polyubquitinated complex is then recognized by a complex of degradative enzymes which together form the proteosome. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions seen in serious human disease states which appear to be resistant to normal degradation, so that ubiquitin antibodies are very useful for studies of these inclusions. For example the neurofibrillary tangles and paired helical filaments of Alzheimer's disease, the Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can all be readily visualized with ubiquitin antibodies of appropriate specificity.
Product was raised against purified ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin. This antibody can used to study ubiquitinated inclusions seen in Alzheimer's and other kinds of disease; such as such as the Lewy bodies of Parkinson's disease and the Pick bodies in Pick's disease in formalin fixed tissues. It can be used on mildly fixed histological sections of human brain, and paraffin embedded material.

NCBI and Uniprot Product Information

• NCBI GI #: 340068
• NCBI GeneID: 7316
• Molecular Weight: 77,039 Da
• NCBI Official Full Name: ubiquitin
• NCBI Official Synonym Full Names: ubiquitin C
• NCBI Official Symbol: UBC
• NCBI Official Synonym Symbols: HMG20
• NCBI Protein Information: polyubiquitin-C
• UniProt Protein Name: Polyubiquitin-C
• Protein Family: Ubiquitin
• UniProt Gene Name: UBC

NCBI Description

This gene represents a ubiquitin gene, ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways. [provided by RefSeq, Aug 2010]

Uniprot Description

UBC: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Belongs to the ubiquitin family.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cytosol; endoplasmic reticulum membrane; endosome membrane; extracellular space; mitochondrial outer membrane; nucleoplasm; nucleus; plasma membrane; vesicle

Molecular Function: protease binding; protein binding; RNA binding

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; bypass DNA synthesis; cellular iron ion homeostasis; cellular protein metabolic process; DNA damage response, detection of DNA damage; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; endosome transport; error-prone postreplication DNA repair; fibroblast growth factor receptor signaling pathway; G2/M transition of mitotic cell cycle; glycogen biosynthetic process; I-kappaB kinase/NF-kappaB cascade; innate immune response; JNK cascade; macroautophagy; MAPKKK cascade; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of interferon type I production; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; Notch signaling pathway; nucleotide-excision repair, DNA damage recognition; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; positive regulation of apoptosis; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of transcription from RNA polymerase II promoter; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; protein folding; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of interferon type I production; regulation of mRNA stability; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; T cell receptor signaling pathway; transcription-coupled nucleotide-excision repair; transforming growth factor beta receptor signaling pathway; transmembrane transport; tumor necrosis factor-mediated signaling pathway; viral infectious cycle; virus assembly; Wnt receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway