Inhibition Assay
(Inhibition Assay: HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with U1000-06Q (1:500) (L1) preincubated with 0.08ug/ml (L2), 0.8ug/ml (L3) or 8ug/ml (L4) of K63-linked multiubiquitin chains. Proteins were visualized using Rb xDk IgG (HRP) and a chemiluminescent detection.)
Rabbit Ubiquitin Monoclonal Antibody | anti-UBA52 antibody
Ubiquitin (Lys63) (MaxLight 405)
Inhibition Assay
(Inhibition Assay: HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with U1000-06Q (1:500) (L1) preincubated with 0.08ug/ml (L2), 0.8ug/ml (L3) or 8ug/ml (L4) of K63-linked multiubiquitin chains. Proteins were visualized using Rb xDk IgG (HRP) and a chemiluminescent detection.)
Inhibition Assay
(Inhibition Assay: HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with U1000-06Q (1:500) (L1) preincubated with 0.08ug/ml (L2), 0.8ug/ml (L3) or 8ug/ml (L4) of K63-linked multiubiquitin chains. Proteins were visualized using Rb xDk IgG (HRP) and a chemiluminescent detection.)
Testing Data
(300ng each of free ubiquitin (L1), K48- linked multiubiquitin chains (L2) and K63-linked multiubiquitin chains (L3) were resolved by electrophoresis, transferred to PVDF and probed with U1000-06Q (1:1000). Proteins were visualized using a Rb xDk IgG (HRP) and a chemiluminescence detection system.)
Testing Data
(300ng each of free ubiquitin (L1), K48- linked multiubiquitin chains (L2) and K63-linked multiubiquitin chains (L3) were resolved by electrophoresis, transferred to PVDF and probed with U1000-06Q (1:1000). Proteins were visualized using a Rb xDk IgG (HRP) and a chemiluminescence detection system.)
Immunoprecipitation (IP)
(100ug of HeLa cell lysate was precleared with 5ug of rabbit IgG and immunoprecipitated with 15ul of U1000-06Q Immunoprecipitated complex was captured by Protein A agarose beads, separated on SDS-PAGE gel and transferred to PVDF membrane. A 1:500 dilution of U1000-06Q was used to detect the ubiquitin complex by Western Blot.)
Immunoprecipitation (IP)
(100ug of HeLa cell lysate was precleared with 5ug of rabbit IgG and immunoprecipitated with 15ul of U1000-06Q Immunoprecipitated complex was captured by Protein A agarose beads, separated on SDS-PAGE gel and transferred to PVDF membrane. A 1:500 dilution of U1000-06Q was used to detect the ubiquitin complex by Western Blot.)
Flow Cytometry (FC/FACS)
(Flow Cytometry: Fixed and permeabilized Jurkat cells were labeled with 1:40 dilution of U1000-06Q or control rabbit IgG, and then stained with Dk xRb IgG (PE).)
Flow Cytometry (FC/FACS)
(Flow Cytometry: Fixed and permeabilized Jurkat cells were labeled with 1:40 dilution of U1000-06Q or control rabbit IgG, and then stained with Dk xRb IgG (PE).)
Immunohistochemistry (IHC)
(Formalin fixed colorectal carcinoma sections were heated in citrate buffer, pH 6 for antigen retrieval, then incubated with U1000-06Q (1:20), followed by biotinylated anti-rabbit secondary, streptavidin-HRP, and DAB.)
Immunohistochemistry (IHC)
(Formalin fixed colorectal carcinoma sections were heated in citrate buffer, pH 6 for antigen retrieval, then incubated with U1000-06Q (1:20), followed by biotinylated anti-rabbit secondary, streptavidin-HRP, and DAB.)
NCBI and Uniprot Product Information
NCBI Description
Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008]
Uniprot Description
UBA52: the gene (UBA52) that encodes this protein is one of four that encode for ubiquitin: UBC, UBB, UBA52 and RPS27A. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. The RPS27A gene product is cleaved into the following 2 chains: ubiquitin (amino acids 1-76) and the 60S ribosomal protein L40 (77 ? 128). Ubiquitin is a peptide 76 amino acids in length that can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Hundreds of ubiquitin ligases and hydrolases have been identified, implicating ubiquitin as a major regulatory element in many crucial cellular systems. It can be covalently bound to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. At the protein level, it is not possible to easily determine which of the four genes encoded a given ubiquitin chain.
Protein type: Ribosomal; Translation
Chromosomal Location of Human Ortholog: 19p13.1-p12
Cellular Component: nucleoplasm; lysosomal membrane; plasma membrane; ribosome; endosome membrane; cytosol
Molecular Function: protein binding; structural constituent of ribosome
Biological Process: circadian rhythm; I-kappaB kinase/NF-kappaB cascade; SRP-dependent cotranslational protein targeting to membrane; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of apoptosis; activation of MAPK activity; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; endosome transport; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of apoptosis; toll-like receptor 5 signaling pathway; antigen processing and presentation of peptide antigen via MHC class I; transforming growth factor beta receptor signaling pathway; translational initiation; JNK cascade; antigen processing and presentation of exogenous peptide antigen via MHC class I; viral transcription; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; regulation of interferon type I production; glycogen biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; fibroblast growth factor receptor signaling pathway; transcription, DNA-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; Notch receptor processing; glucose metabolic process; virus assembly; toll-like receptor 2 signaling pathway; translational elongation; carbohydrate metabolic process; viral protein processing; mRNA catabolic process, nonsense-mediated decay; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of interferon type I production; negative regulation of apoptosis; G1/S transition of mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of epidermal growth factor receptor signaling pathway; translation; apoptosis; pathogenesis; viral infectious cycle; negative regulation of transcription from RNA polymerase II promoter; translational termination; toll-like receptor 10 signaling pathway; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of interferon type I production; transmembrane transport; epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; protein modification process; DNA repair; MyD88-dependent toll-like receptor signaling pathway; cellular protein metabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; toll-like receptor signaling pathway; innate immune response; gene expression; mitotic cell cycle; negative regulation of transforming growth factor beta receptor signaling pathway
Research Articles on UBA52
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Product Notes
The UBA52 (Catalog #AAA6245130) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ubiquitin (Lys63) (MaxLight 405) reacts with Human, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ubiquitin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the UBA52 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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