Mouse UBE2C Monoclonal Antibody | anti-UBE2C antibody

UBE2C Antibody (C-term)

Cat. Num. AAA9231329

• Gene Names: UBE2C; UBCH10; dJ447F3.2
• Reactivity: Human; Predicted: Bovine, Monkey, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified through a protein G column, followed by dialysis against PBS.
• Synonyms: UBE2C; Monoclonal Antibody; UBE2C Antibody (C-term); Ubiquitin-conjugating enzyme E2 C; 6.3.2.19; UbcH10; Ubiquitin carrier protein C; Ubiquitin-protein ligase C; UBCH10; anti-UBE2C antibody

• Host: Mouse
• Reactivity: Human; Predicted: Bovine, Monkey, Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 2001CT211.37.61
• Specificity: This UBE2C antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 137~172 amino acids from the C-terminal region of human UBE2C.
• Purity/Purification: Purified through a protein G column, followed by dialysis against PBS.
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 132

• Applicable Applications for anti-UBE2C antibody: Western Blot (WB), ELISA (EIA)
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Dilution: WB - 1:2000All lanes: Anti-UBE2C Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: A431 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-UBE2C antibody

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.

Product Categories/Family for anti-UBE2C antibody

Cell Biology

References

Tortorella M.D.,et al.Science 284:1664-1666(1999). Wainwright S.D.,et al.Matrix Biol. 25:317-320(2006). Sawaji Y.,et al.Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases. Ishikawa K.,et al.DNA Res. 5:169-176(1998). Clark H.F.,et al.Genome Res. 13:2265-2270(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 528524529
• NCBI GeneID: 11065
• NCBI Accession #: NP_001268670.1
• NCBI GenBank Nucleotide #: NM_001281741.1
• UniProt Accession #: O00762; Q32PA5; Q4R9D1; Q9D1C1
• Molecular Weight: 19652 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 C isoform 6
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 C
• NCBI Official Symbol: UBE2C
• NCBI Official Synonym Symbols: UBCH10; dJ447F3.2
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 C
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 C
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2C
• UniProt Synonym Gene Names: UBCH10
• UniProt Entry Name: UBE2C_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013]

Uniprot Description

UBE2C: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ubiquitin conjugating system; Ligase; Cell cycle regulation; EC 6.3.2.19; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: nucleoplasm; anaphase-promoting complex; cytoplasm; cytosol

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; protein ubiquitination; cyclin catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex activation; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; exit from mitosis; positive regulation of exit from mitosis