Mouse UbcH1, E2-25K Monoclonal Antibody
UbcH1, E2-25K (Huntingtin Interacting Protein 2, HIP2)
Applications
Western Blot, Gel Super Shift Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
UbcH1; E2-25K; Monoclonal Antibody; E2-25K (Huntingtin Interacting Protein 2; HIP2); Anti -UbcH1; anti-UbcH1; E2-25K antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C346
Specificity
Recognizes human UbcH1, E2-25K.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Applicable Applications for anti-UbcH1, E2-25K antibody
Western Blot (WB), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in Dot Blot and Western Blot.
Immunogen
Partial sequence of recombinant full-length human UbcH1, E2-25K
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-UbcH1, E2-25K antibody
Protein ubiquitinylation requires the concerted action of the E1, E2 and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through an ATP-dependent formation of a thiol ester with E1. The activated ubiquitin is then transferred to a thiol-group of ubiquitin-conjugation enzyme E2. The final step is the transfer of ubiquitin from E2 to an e-amino group of the target protein lysine residue, which is mediated by ubiuitin-conjugation enzyme E3. E2-25K (Hip2) is a member of the E2 protein family that catalyzes multiubiquitin chain synthesis via Lys48 of ubiquitin. E2-25K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-b, huntingtin, and MHC-heavy chain proteins. Lys14 of E2-25K can be modified by SUMOylation, with this modification resulting in inhibited E2 activity.
Product Categories/Family for anti-UbcH1, E2-25K antibody
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Product Notes
The UbcH1, E2-25K (Catalog #AAA603135) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's UbcH1, E2-25K can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Gel Shift Assay (GS/EMSA). Suitable for use in Dot Blot and Western Blot. Researchers should empirically determine the suitability of the UbcH1, E2-25K for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UbcH1, E2-25K, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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