Testing Data
(Image: TH Staining in the Substantia Nigra.)
Mouse anti-Human Tyrosine Hydroxylase Monoclonal Antibody | anti-TH antibody
Tyrosine Hydroxylase, Monoclonal Antibody, Mouse
Western Blot: 1:1000-1:2000
Note: It is strongly recommended that the customer perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system as used in our protocol may change the configuration of the protein and, therefore, may alter the reactivity with the tissue tested.
Application Notes for Immunohistochemistry
Control Tissue: Rat striatum and substantia nigra
Perfusion Fixation: Fixative - 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4. Post Fixation - 1.5 hr. at 4° C in 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4.
Sections: 10 um cryostat frozen sections or 50 ?m vibratome sections.
Dilution recommendation: 1/1,000 – 1/2,000 in PBS/0.3% Triton X-100 Cy3 Technique 1/4,000 - 1/8,000 in PBS/0.3% Triton X-100 Bn-Av/HRP Technique
Incubation on tissue: 18-24 hr at 2-8 degree C
Detection System: Use Cy3 and Bn-Av/HRP reagents at dilutions recommended by the manufacturer.
Storage after reconstitution: Dilute with phosphate buffer or Tris buffer at dilutions no higher than 1/10, aliquot and freeze at -15 degree C or lower.
Stability after reconstitution: Antibody can be stored for up to six months if handled as described above.
Testing Data
(Image: TH Staining in the Substantia Nigra.)
Testing Data
(Image: TH Staining in the Substantia Nigra.)
This Tyrosine Hydroxylase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat catecholamine neuron systems using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions for these methods are provided on the data-sheet. This antibody does not cross react with dihydropterdine reductase, dopamine-B-hydroxylase, phenylethanolamine-N-methyltransferase, phenylalanine hydroxylase or tryptophan hydroxylase using Western blot methods.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.
Protein type: EC 1.14.16.2; Vesicle; Mitochondrial; Amino Acid Metabolism - tyrosine; Endoplasmic reticulum; Oxidoreductase
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: internal side of plasma membrane; neuron projection; smooth endoplasmic reticulum; mitochondrion; melanosome membrane; dendrite; terminal button; perikaryon; cytosol; synaptic vesicle; cytoplasm; cytoplasmic vesicle; nucleus
Molecular Function: amino acid binding; protein domain specific binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity
Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; neurotransmitter biosynthetic process; response to corticosterone stimulus; response to pyrethroid; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; organ morphogenesis; eye photoreceptor cell development; response to ethanol; circadian sleep/wake cycle; response to zinc ion; response to activity; cerebral cortex development; response to water deprivation; response to peptide hormone stimulus; synaptic transmission, dopaminergic; locomotory behavior; fatty acid metabolic process; response to salt stress; response to estradiol stimulus; regulation of heart contraction; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; learning; response to herbicide; response to ether; synaptic vesicle amine transport; memory; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis
Disease: Segawa Syndrome, Autosomal Recessive
Research Articles on TH
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Product Notes
The TH th (Catalog #AAA555025) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Tyrosine Hydroxylase, Monoclonal Antibody, Mouse reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tyrosine Hydroxylase can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Immunohistochemistry: 1:1000-1:2000 in PBS/0.3% Triton X-100 Cy3 Technique 1:4000-1:8000 in PBS/0.3% Triton X-100 Bn-Av/HRP Technique Western Blot: 1:1000-1:2000 Note: It is strongly recommended that the customer perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system as used in our protocol may change the configuration of the protein and, therefore, may alter the reactivity with the tissue tested. Application Notes for Immunohistochemistry Control Tissue: Rat striatum and substantia nigra Perfusion Fixation: Fixative - 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4. Post Fixation - 1.5 hr. at 4° C in 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4. Sections: 10 um cryostat frozen sections or 50 ?m vibratome sections. Dilution recommendation: 1/1,000 – 1/2,000 in PBS/0.3% Triton X-100 Cy3 Technique 1/4,000 - 1/8,000 in PBS/0.3% Triton X-100 Bn-Av/HRP Technique Incubation on tissue: 18-24 hr at 2-8 degree C Detection System: Use Cy3 and Bn-Av/HRP reagents at dilutions recommended by the manufacturer. Researchers should empirically determine the suitability of the TH th for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tyrosine Hydroxylase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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