Rabbit anti-Human PAK1 Polyclonal Antibody | anti-PAK1 antibody

PAK1 Rabbit Polyclonal

Cat. Num. AAA7602353

• Gene Names: PAK1; PAKalpha
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: PAK1; Polyclonal Antibody; PAK1 Rabbit Polyclonal; anti-PAK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 545

• Applicable Applications for anti-PAK1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: P21 protein (Cdc42/Rac)-activated kinase 1
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer slide using MBS7602353 (PAK1 Antibody) at dilution of 1:50)

Western Blot (WB)

(Jurkat cells were subjected to SDS PAGE followed by western blot with MBS7602353 (PAK1 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 1256422
• NCBI GeneID: 5058
• Molecular Weight: 61,632 Da
• NCBI Official Full Name: PAK1
• NCBI Official Synonym Full Names: p21 (RAC1) activated kinase 1
• NCBI Official Symbol: PAK1
• NCBI Official Synonym Symbols: PAKalpha
• NCBI Protein Information: serine/threonine-protein kinase PAK 1
• UniProt Protein Name: Serine/threonine-protein kinase PAK 1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK1
• UniProt Synonym Gene Names: PAK-1
• UniProt Entry Name: PAK1_HUMAN

NCBI Description

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Uniprot Description

PAK1: a protein kinase of the STE20 family that regulates cell motility and morphology. Critical RhoGTPase effector that regulates cytoskeleton reorganization, the JNK MAPK pathway, and nuclear signaling. Binding of Rac/cdc42 to the PBD of PAK1 causes autophosphorylation and conformational change. Phosphorylated and activated by PDK.

Protein type: Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; STE group; STE20 family; PAKA subfamily

Chromosomal Location of Human Ortholog: 11q13-q14

Cellular Component: axon; cytoplasm; cytosol; dendrite; filamentous actin; focal adhesion; Golgi apparatus; intercellular junction; nuclear membrane; plasma membrane; protein complex; ruffle; Z disc

Molecular Function: ATP binding; collagen binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: actin cytoskeleton reorganization; apoptosis; axonogenesis; branching morphogenesis of a tube; cellular response to insulin stimulus; ephrin receptor signaling pathway; exocytosis; MAPKKK cascade; mitotic cell cycle; neurite morphogenesis; positive regulation of cell migration; positive regulation of estrogen receptor signaling pathway; positive regulation of JNK activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of stress fiber formation; protein amino acid autophosphorylation; protein amino acid phosphorylation; response to hypoxia; stimulatory C-type lectin receptor signaling pathway; T cell costimulation; T cell receptor signaling pathway; wound healing