Western Blot (WB)
(Western blot analysis of twist expression in non-transfected (A) and human twist transfected (B) 293T whole cell lysates.)
Mouse TWIST Monoclonal Antibody | anti-TWIST antibody
TWIST Antibody
Gene Names
TWIST1; CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
TWIST; Monoclonal Antibody; TWIST Antibody; ACS3 antibody; B-HLH DNA binding protein antibody; bHLHa38 antibody; BPES2 antibody; BPES3 antibody; Class A basic helix-loop-helix protein 38 antibody; CRS1 antibody; H-twist antibody; OTTHUMP00000116043 antibody; SCS antibody; TWIST antibody; Twist basic helix loop helix transcription factor 1 antibody; Twist homolog 1 (Drosophila) antibody; Twist homolog 1 antibody; TWIST homolog of drosophila antibody; Twist related protein 1 antibody; Twist-related protein 1 antibody; TWIST1 antibody; TWST1_HUMAN antibody; anti-TWIST antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
1G3
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-TWIST antibody
Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:100-1:1000
IP: 1-2ug per 100-500ug of total protein (1 ml of cell lysate)
FC/FACS: 1ug per 1 x 106 cells
IP: 1-2ug per 100-500ug of total protein (1 ml of cell lysate)
FC/FACS: 1ug per 1 x 106 cells
Immunogen
A Recombinant protein corresponding to a region near the C-terminus of twist of human origin.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of twist expression in non-transfected (A) and human twist transfected (B) 293T whole cell lysates.)
Western Blot (WB)
(Western blot analysis of twist expression in non-transfected (A) and human twist transfected (B) 293T whole cell lysates.)
Related Product Information for anti-TWIST antibody
Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes. Class A proteins include the ubiquitously expressed E-box binding factors E12/E47, ITF2 and HEB (BETA1 or HTF4). Class B proteins such as MyoD, myogenin and NeuroD (BETA2) are transiently expressed and exhibit a much more limited tissue distribution. Class A proteins heterodimerize with class B proteins to activate DNA transcription. Working in opposition to these positively acting factors are a specialized group of proteins that function as dominant negative regulators. Muscle tissue is derived from a subset of cells originating from the embryonic mesoderm. The novel basic helix-loop-helix (bHLH) transcription factor twist is a putative regulator of mesodermal differentiation and myogenesis. Twist is expressed throughout the epithelial somite but not in the myotome. Twist requires dimerization with the E proteins and inhibits myogenic regulatory factors. It has been implicated as regulator of the temporal and spatial formation of myotomes.
Product Categories/Family for anti-TWIST antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,954 Da
NCBI Official Full Name
Homo sapiens twist family bHLH transcription factor 1 (TWIST1), mRNA
NCBI Official Synonym Full Names
twist family bHLH transcription factor 1
NCBI Official Symbol
TWIST1
NCBI Official Synonym Symbols
CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
NCBI Protein Information
twist-related protein 1; B-HLH DNA binding protein; H-twist; TWIST homolog of drosophila; class A basic helix-loop-helix protein 38; twist basic helix-loop-helix transcription factor 1; twist homolog 1
UniProt Protein Name
Twist-related protein 1
Protein Family
UniProt Gene Name
TWIST1
UniProt Synonym Gene Names
BHLHA38; TWIST; bHLHa38
UniProt Entry Name
TWST1_HUMAN
NCBI Description
Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq, Jul 2008]
Research Articles on TWIST
Similar Products
Product Notes
The TWIST twist1 (Catalog #AAA9435199) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TWIST Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TWIST can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:100-1:1000 IP: 1-2ug per 100-500ug of total protein (1 ml of cell lysate) FC/FACS: 1ug per 1 x 106 cells. Researchers should empirically determine the suitability of the TWIST twist1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TWIST, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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