Galactose-3-O-Sulfotransferase 1 (GAL3ST1) Recombinant Protein | GAL3ST1 recombinant protein

Recombinant Galactose-3-O-Sulfotransferase 1 (GAL3ST1)

Cat. Num. AAA2018501

• Gene Names: GAL3ST1; CST
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Galactose-3-O-Sulfotransferase 1 (GAL3ST1); Recombinant Galactose-3-O-Sulfotransferase 1 (GAL3ST1); GAL3ST1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-LEPE AVIRANGSAG ECQPRRNIVF LKTHKTASST LLNILFRFGQ KHRLKFAFPN GRNDFDYPTF FARSLVQDYR PGACFNIICN HMRFHYDEVR GLVPTNAIFI TVLRDPARLF ESSFHYFGPVVPLTWKLSAG DKLTEF
• Sequence Length: 423

• Applicable Applications for GAL3ST1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Leu57~Phe196 (Accession # Q99999) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Golgi apparatus membrane; Single-pass type II membrane protein
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4758088
• NCBI GeneID: 9514
• NCBI Accession #: NP_004852.1
• NCBI GenBank Nucleotide #: NM_004861.1
• UniProt Accession #: Q99999; Q96C63
• Molecular Weight: 48.5kDa
• NCBI Official Full Name: galactosylceramide sulfotransferase
• NCBI Official Synonym Full Names: galactose-3-O-sulfotransferase 1
• NCBI Official Symbol: GAL3ST1
• NCBI Official Synonym Symbols: CST
• NCBI Protein Information: galactosylceramide sulfotransferase; GalCer sulfotransferase; cerebroside sulfotransferase; 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase; 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase; cerebroside (3'-phosphoadenylylsulfate:galactosylceramide 3') sulfotransferase
• UniProt Protein Name: Galactosylceramide sulfotransferase
• Protein Family: Galactosylceramide sulfotransferase
• UniProt Gene Name: GAL3ST1
• UniProt Synonym Gene Names: CST; GalCer sulfotransferase
• UniProt Entry Name: G3ST1_HUMAN

NCBI Description

Sulfonation, an important step in the metabolism of many drugs, xenobiotics, hormones, and neurotransmitters, is catalyzed by sulfotransferases. The product of this gene is galactosylceramide sulfotransferase which catalyzes the conversion between 3'-phosphoadenylylsulfate + a galactosylceramide to adenosine 3',5'-bisphosphate + galactosylceramide sulfate. Activity of this sulfotransferase is enhanced in renal cell carcinoma. [provided by RefSeq, Jul 2008]

Uniprot Description

GAL3ST1: Catalyzes the sulfation of membrane glycolipids. Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety. Catalyzes the synthesis of galactosylceramide sulfate (sulfatide), a major lipid component of the myelin sheath and of monogalactosylalkylacylglycerol sulfate (seminolipid), present in spermatocytes. Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro). Belongs to the galactose-3-O-sulfotransferase family.

Protein type: Membrane protein, integral; Lipid Metabolism - sphingolipid; Transferase; EC 2.8.2.11

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: Golgi membrane; membrane; integral to plasma membrane

Molecular Function: sulfotransferase activity; galactosylceramide sulfotransferase activity

Biological Process: myelination; galactosylceramide biosynthetic process; protein amino acid N-linked glycosylation; spermatogenesis