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Immunofluorescence (IF) (Immunofluorescence staining of microtubular networks in 3T3 mouse fibroblasts. 3A - metaphase; 3B - anaphase; 3C - telophase Gamma-tubulin (red) stained with MBS606699, alpha-tubulin (green) with polyclonal anti-alpha-tubulin antibody and nuclei with DAPI (blue).)

Mouse Tubulin, gamma Monoclonal Antibody

Tubulin, gamma (TUBG, Tubulin gamma 1 Chain, TUBG1, Tubulin gamma 2 Chain, TUBG2, Gamma 1 Tubulin, Gamma 2 Tubulin, Gamma Tubulin 1, Gamma Tubulin 2, Gamma Tubulin Complex Component 1, GCP 1, GCP-1, MGC131994, Xgam) (Dyomics 547)

Reactivity
Chicken, Human, Mouse, Porcine, Rat, Xenopus
Applications
Immunocytochemistry
Purity
Purified
Purified
Synonyms
Tubulin; gamma; Monoclonal Antibody; gamma (TUBG; Tubulin gamma 1 Chain; TUBG1; Tubulin gamma 2 Chain; TUBG2; Gamma 1 Tubulin; Gamma 2 Tubulin; Gamma Tubulin 1; Gamma Tubulin 2; Gamma Tubulin Complex Component 1; GCP 1; GCP-1; MGC131994; Xgam) (Dyomics 547); Anti -Tubulin; anti-Tubulin; gamma antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Chicken, Human, Mouse, Porcine, Rat, Xenopus
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
9F154
Specificity
Recognizes human gamma tubulin in stomach, kidney, liver, lung, muscle, ovary, heart, colon, thymus, brain
Purity/Purification
Purified
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Dyomics 547.
Applicable Applications for anti-Tubulin, gamma antibody
Immunocytochemistry (ICC)
Application Notes
Suitable for use in Immunocytochemistry.
Dilution: Immunocytochemistry: 1:500. Samples need to be fixed and permeabilized
Immunogen
Synthetic peptide corresponding to aa434-449, EYHAATRPDYISWGTQ representing the C-Terminus of the human protein of gamma Tubulin (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Dyomics 547 conjugates are sensitive to light.

Immunofluorescence (IF)

(Immunofluorescence staining of microtubular networks in 3T3 mouse fibroblasts. 3A - metaphase; 3B - anaphase; 3C - telophase Gamma-tubulin (red) stained with MBS606699, alpha-tubulin (green) with polyclonal anti-alpha-tubulin antibody and nuclei with DAPI (blue).)

Immunofluorescence (IF) (Immunofluorescence staining of microtubular networks in 3T3 mouse fibroblasts. 3A - metaphase; 3B - anaphase; 3C - telophase Gamma-tubulin (red) stained with MBS606699, alpha-tubulin (green) with polyclonal anti-alpha-tubulin antibody and nuclei with DAPI (blue).)

Immunofluorescence (IF)

(Immunofluorescence staining of mouse fibroblasts MBS606699 (direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).)

Immunofluorescence (IF) (Immunofluorescence staining of mouse fibroblasts MBS606699 (direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).)

Immunofluorescence (IF)

(Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using MBS606699 (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).)

Immunofluorescence (IF) (Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using MBS606699 (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).)
Related Product Information for anti-Tubulin, gamma antibody
Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end.
Product Categories/Family for anti-Tubulin, gamma antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI Official Full Name
tubulin

Uniprot Description

TUBG1: Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Interacts with GCP2 and GCP3. Interacts with B9D2. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO/C1orf88. Belongs to the tubulin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Cellular Component: centrosome; pericentriolar material; condensed nuclear chromosome; nonmotile primary cilium; leading edge; polar microtubule; cilium; gamma-tubulin complex; centriole; recycling endosome; cytoplasmic microtubule; apical part of cell; spindle microtubule; cytoplasm; microtubule organizing center

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: metabolic process; microtubule nucleation; meiotic spindle organization and biogenesis; cytoplasmic microtubule organization and biogenesis

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Product Notes

The Tubulin, gamma (Catalog #AAA606699) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Tubulin, gamma (TUBG, Tubulin gamma 1 Chain, TUBG1, Tubulin gamma 2 Chain, TUBG2, Gamma 1 Tubulin, Gamma 2 Tubulin, Gamma Tubulin 1, Gamma Tubulin 2, Gamma Tubulin Complex Component 1, GCP 1, GCP-1, MGC131994, Xgam) (Dyomics 547) reacts with Chicken, Human, Mouse, Porcine, Rat, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Tubulin, gamma can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC). Suitable for use in Immunocytochemistry. Dilution: Immunocytochemistry: 1:500. Samples need to be fixed and permeabilized. Researchers should empirically determine the suitability of the Tubulin, gamma for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tubulin, gamma, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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