Testing Data
Hamster anti-Mouse CD3e Monoclonal Antibody | anti-CD3e antibody
Anti-Mouse CD3e, Biotin (Clone 145-2C11) (hamster IgG)
Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
Synonyms
CD3e; Monoclonal Antibody; Anti-Mouse CD3e; Biotin (Clone 145-2C11) (hamster IgG); Biotin Anti-Mouse CD3e Monoclonal Antibody; anti-CD3e antibody
Host
Hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Hamster IgG
Clone Number
145-2C11
Specificity
CD3e (Mouse CD3epsilon)
Form/Format
Biotin
Concentration
Antibody Concentration: 0.1 mg/ml (varies by lot)
Sequence Length
198
Applicable Applications for anti-CD3e antibody
Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)
Immunogen
H-2Kb sp
Donor
Armenian Hamster Spleen
Fusion Partner
Murine myeloma cell line SP2/0
Presentation
100 ug or 300 ug biotin-labelled Ig buffered in PBS + 0.02% NaN3. BSA was added to bring final protein concentration to 4-5 mg/ml.
Preparation and Storage
Stable at 4 degree C. For long term storage, aliquot and freeze unused portions at -20 degree C in volumes appropriate for single usage. Avoid repeated freeze/thaw cycles.
Testing Data
Testing Data
Related Product Information for anti-CD3e antibody
anti-mouse CD3e monoclonal antibody is specific for a 25 kDa protein component (e-T3) of the antigen specific T cell receptor on all mouse strains tested. The e-T3 protein has been shown to be non-covalently associated on the cell surface ab heterodimer of the CD3 associated complex. This monoclonal antibody reacts with all mature T cells and can both activate and inhibit T cell function (1). This fact identifies e-T3 as a cell surface protein involved in the transduction of activation signals. All peripheral T cells express this determinant however B cells and bone marrow cells have proven to be negative. Although the expression of this particular epitope on peripheral T cells is uniformly high, staining of thymocytes reveals distinct subpopulations of cells differing in the level of expression of this marker. This antibody will prove useful in studying the role of various components of the TCR complex in T cell activation and development, and will allow for the development of an animal model in which to investigate the immunoregulatory effects of in vivo administration of anti-CD3 antibodies, an area of obvious clinical importance. Anti-CD3e is ideal for flow cytometry applications, particularly as a specific marker for tracking mouse T cells. In addition, this monoclonal antibody, clone 145-2C11, was specifically designed to trigger T cell activation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
CD3e molecule, epsilon (CD3-TCR complex)
Protein Family
Similar Products
Product Notes
The CD3e (Catalog #AAA520096) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD3e, Biotin (Clone 145-2C11) (hamster IgG) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD3e can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3e, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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