Mouse anti-Human Troponin I, Cardiac, aa87-91 Monoclonal Antibody | anti-TNNT2 antibody

Mouse anti-Human Troponin I, Cardiac, aa87-91 (cTnl)

Cat. Num. AAA6000478

• Gene Names: TNNT2; CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: >90% Purified by Protein A affinity chromatography. Purity is tested by electrophoresis.
• Synonyms: Troponin I; Cardiac; aa87-91; Monoclonal Antibody; Mouse anti-Human Troponin I; aa87-91 (cTnl); Anti -Troponin I; aa87-91 (cTnI; CMH7; Familial Hypertrophic Cardiomyopathy 7; MGC116817; TNNC1; Troponin I Type 3 Cardiac; TNNI3); anti-TNNT2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1.BB.146
• Specificity: Recognizes free human cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. Not affected by heparin, phosphorylation and oxidation. Recognizes an epitope located between amino acid residues 87 and 91 of cTnI. No crossreactivity with skeletal muscle troponin I. Species Crossreactivity: bovine, porcine, goat, canine, rabbit and feline. Not reactive with cTnl from rat, mouse or fish.
• Purity/Purification: >90% Purified by Protein A affinity chromatography. Purity is tested by electrophoresis.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
• Concentration: 4.7 mg/mL (varies by lot)

• Applicable Applications for anti-TNNT2 antibody: ELISA, Western Blot (WB), Immunoaffinity Purification
• Application Notes: Other applications not tested. Optimal dilutions to be determined by the researcher.
• Immunogen: Free human cardiac troponin I.
• Grade: Affinity Purified
• Hybridoma: SP2/0 myeloma cells with spleen cells from Balb/c mice.
• Preparation and Storage: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-TNNT2 antibody

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter "I" is given due to its inhibitory character.

Product Categories/Family for anti-TNNT2 antibody

Antibodies; Abs to Cardiac Markers

NCBI and Uniprot Product Information

• NCBI GI #: 48255883
• NCBI GeneID: 7139
• NCBI Accession #: NP_001001432.1
• NCBI GenBank Nucleotide #: NM_001001432.2
• UniProt Accession #: P45379; O60214; Q99596; Q99597; Q9BUF6; Q9UM96; A2TDB9; A8K3K6
• NCBI Official Full Name: troponin T, cardiac muscle isoform 4
• NCBI Official Synonym Full Names: troponin T type 2 (cardiac)
• NCBI Official Symbol: TNNT2
• NCBI Official Synonym Symbols: CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6
• NCBI Protein Information: troponin T, cardiac muscle; troponin T2, cardiac; cardiomyopathy, hypertrophic 2; cardiomyopathy, dilated 1D (autosomal dominant)
• UniProt Protein Name: Troponin T, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNT2
• UniProt Synonym Gene Names: TnTc; cTnT
• UniProt Entry Name: TNNT2_HUMAN

NCBI Description

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: sarcomere; troponin complex; cytosol; striated muscle thin filament

Molecular Function: troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; actin binding; tropomyosin binding

Biological Process: metabolic process; atrial cardiac muscle morphogenesis; regulation of muscle contraction; positive regulation of ATPase activity; ventricular cardiac muscle morphogenesis; sarcomere organization; response to calcium ion; negative regulation of ATPase activity; regulation of heart contraction; muscle filament sliding

Disease: Cardiomyopathy, Familial Restrictive, 3; Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2