anti-Human Troponin I-Cardiac (25-40 Region) Monoclonal Antibody | anti-TNNI3 antibody

MAb to Troponin I-Cardiac

Cat. Num. AAA311165

• Gene Names: TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; MGC116817
• Reactivity: Human
• Purity: >90% pure (SDS-PAGE). Protein A Sepharose chromatography.
• Synonyms: Troponin I-Cardiac (25-40 Region); Monoclonal Antibody; MAb to Troponin I-Cardiac; Monoclonal Antibody to Human Cardiac Troponin I (cTnI); anti-TNNI3 antibody

• Host: Host: Mouse. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.; Source: Ascites
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3C7
• Specificity: Troponin I-Cardiac (25-40 Region); Reacts equally with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. Not affected by heparin, phosphorylation and oxidation. Recognizes an epitope located between amino acid residues 25 and 40 of cTnI. No cross-reactivity with skeletal muscle troponin I.
• Purity/Purification: >90% pure (SDS-PAGE). Protein A Sepharose chromatography.
• Form/Format: Biotin, Liquid
• Concentration: 0.9mg/ml (Sigma protein assay kit) (varies by lot)
• Sequence Length: 210

• Immunogen: Native troponin complex
• Affinity Constant: Not determined
• Buffer: 20mM Tris-HCl, pH 7.5 containing 150mM Sodium chloride
• Preservative: 0.1% Sodium azide
• Important Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
• Preparation and Storage: Store at 2 to 8 degree C.

Product Categories/Family for anti-TNNI3 antibody

Monoclonal Antibodies to Cardiac Markers

NCBI and Uniprot Product Information

• NCBI GI #: 151101270
• NCBI GeneID: 7137
• NCBI Accession #: NP_000354.4
• NCBI GenBank Nucleotide #: NM_000363.4
• UniProt Accession #: P19429; Q6FGU5; Q6FGX2
• Molecular Weight: 24,008 Da
• NCBI Official Full Name: troponin I, cardiac muscle
• NCBI Official Synonym Full Names: troponin I type 3 (cardiac)
• NCBI Official Symbol: TNNI3
• NCBI Official Synonym Symbols: CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; MGC116817
• NCBI Protein Information: troponin I, cardiac muscle
• UniProt Protein Name: Troponin I, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNI3
• UniProt Synonym Gene Names: TNNC1
• UniProt Entry Name: TNNI3_HUMAN

NCBI Description

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

Uniprot Description

Function: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Subunit structure: Binds to actin and tropomyosin. Interacts with TRIM63. Ref.7

Involvement in disease: Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [

MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Ref.10 Ref.11 Ref.13 Ref.15 Ref.17Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [

MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [

MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Ref.16Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [

MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Sequence similarities: Belongs to the troponin I family.