Rat anti-Mouse TREM-2 Monoclonal Antibody | anti-TREM-2 antibody

RAT ANTI MOUSE TREM-2

Cat. Num. AAA224541

• Gene Names: Trem2; TREM-2; Trem2a; Trem2b; Trem2c
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: TREM-2; Monoclonal Antibody; RAT ANTI MOUSE TREM-2; anti-TREM-2 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 78.18
• Specificity: Rat anti Mouse TREM-2 antibody, clone 78.18 recognizes triggering receptor expressed on myeloid cells 2 (TREM-2), an activating receptor of the Ig superfamily. TREM-2 forms a receptor signaling complex with DAP12 to trigger the activation of the immune response in macrophages and dendritic cells. It may be involved in chronic inflammation and may promote the production of constitutive rather than inflammatory chemokines and cytokines. TREM-2 may also have a functional role in osteoclast formation.; ; Stimulation of TREM-2 with Rat anti Mouse Trem-2 antibody, clone 78.18 enhances multinucleated osteoclast formation in vitro. Rat anti Mouse Trem-2 antibody, clone 78.18 may also be used to block TREM-2 on pre-osteoclasts to inhibit the formation of multinuclear osteoclasts and to inhibit bone resorption by mature osteoclasts.
• Form/Format: Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)

• Applicable Applications for anti-TREM-2 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry - ; Suggested Dilution: 1/25-1/200; Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul. ; ; Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
• Target Species: Mouse
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Buffer Solution: Phosphate buffered saline
• Preservative Stabilizers: 0.09% Sodium Azide (NaN₃)
• Carrier Free: Yes
• Immunogen: Mouse TREM-2 Fc fusion protein
• Fusion Partners: Lymph node cells from immunized Fisher rats were fused with cells of the rat YB-2/0 myeloma cell line
• Preparation and Storage: Store at +4°C or at -20°C if preferred.; Storage in frost-free freezers is not recommended.; This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

NCBI and Uniprot Product Information

• NCBI GI #: 440918700
• NCBI GeneID: 83433
• NCBI Accession #: NP_001259007.1
• NCBI GenBank Nucleotide #: NM_001272078.1
• UniProt Accession #: Q99NH8; Q8CGK4; Q8CIA6; Q99NH9; Q9JL34
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 2 isoform 2
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 2
• NCBI Official Symbol: Trem2
• NCBI Official Synonym Symbols: TREM-2; Trem2a; Trem2b; Trem2c
• NCBI Protein Information: triggering receptor expressed on myeloid cells 2; triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2a; triggering receptor expressed on myeloid cells 2b; triggering receptor expressed on myeloid cells 2c
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 2
• UniProt Gene Name: Trem2
• UniProt Synonym Gene Names: Trem2a; Trem2b; Trem2c; TREM-2
• UniProt Entry Name: TREM2_MOUSE

NCBI Description

The protein encoded by this gene is part of the immunoglobulin and lectin-like superfamily and functions as part of the innate immune system. This gene forms part of a cluster of genes on mouse chromosome 17 thought to be involved in innate immunity. This protein associates with the adaptor protein Dap-12 and recruits several factors, such as kinases and phospholipase C-gamma, to form a receptor signaling complex that activates myeloid cells, including dendritic cells and microglia. In humans homozygous loss-of-function mutations in this gene cause Nasu-Hakola disease and mutations in this gene may be risk factors to the development of Alzheimer's disease. In mouse mutations of this gene serve as a pathophysiological model for polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (Nasu-Hakola disease) and for inflammatory bowel disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

Uniprot Description

TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: intracellular membrane-bound organelle; membrane; integral to membrane; plasma membrane; extracellular region

Molecular Function: peptidoglycan binding; protein binding; transmembrane receptor activity; lipopolysaccharide binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; detection of lipopolysaccharide; detection of peptidoglycan; lipopolysaccharide-mediated signaling pathway; innate immune response; positive regulation of calcium-mediated signaling; signal transduction; positive regulation of antigen processing and presentation of peptide antigen via MHC class II