Mouse Transgelin Monoclonal Antibody | anti-TAGLN antibody

Transgelin (SM22-alpha) Mouse Monoclonal Antibody

Cat. Num. AAA438730

• Gene Names: TAGLN; SM22; SMCC; TAGLN1; WS3-10
• Reactivity: Human, Cow, Pig, Rabbit, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: Transgelin; Monoclonal Antibody; Transgelin (SM22-alpha) Mouse Monoclonal Antibody; 2kDa actin-binding protein; Human 22kDa smooth muscle protein; Protein WS3-10; SMCC; Smooth muscle protein 22-alpha (SM22-alpha); TAGLN; TAGLN1; transgelin; Transgelin variant 2; WS3-10 Human Gene SymbolTAGLNHu Chromosome Location11q23.2; anti-TAGLN antibody

• Host: Mouse
• Reactivity: Human, Cow, Pig, Rabbit, Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM606
• Specificity: This MAb recognizes a 22kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 201

• Applicable Applications for anti-TAGLN antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed) (IHC-F)
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Cellular Localization: Cytoplasmic
• Immunogen: Recombinant full-length human transgelin (TAGLN) protein.
• Hu-Chromosome Location: 11q23.2
• Positive Control: U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-paraffin human Colon Carcinoma stained with Transgelin Monoclonal Antibody (SPM606))

References

Shapland, C., et al. 1993. Purification and properties of transgelin: a transformation and shape change sensitive Actin-gelling protein. J. Cell Biol. 121: 1065-1073

NCBI and Uniprot Product Information

• NCBI GI #: 48255907
• NCBI GeneID: 6876
• NCBI Accession #: NP_001001522.1
• NCBI GenBank Nucleotide #: NM_001001522.1
• UniProt Accession #: Q01995; O15542
• Molecular Weight: 22kDa
• NCBI Official Full Name: transgelin
• NCBI Official Synonym Full Names: transgelin
• NCBI Official Symbol: TAGLN
• NCBI Official Synonym Symbols: SM22; SMCC; TAGLN1; WS3-10
• NCBI Protein Information: transgelin
• UniProt Protein Name: Transgelin
• Protein Family: Transgelin
• UniProt Gene Name: TAGLN
• UniProt Synonym Gene Names: SM22; WS3-10; SM22-alpha
• UniProt Entry Name: TAGL_HUMAN

NCBI Description

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TAGLN: Actin cross-linking/gelling protein. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. Belongs to the calponin family.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q23.2

Molecular Function: protein binding

Biological Process: epithelial cell differentiation; muscle development