Mouse TRADD Monoclonal Antibody | anti-TRADD antibody

TRADD (C-terminal region) Antibody

Cat. Num. AAA474617

• Gene Names: TRADD; Hs.89862
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunocytochemistry
• Purity: Protein A chromatography
• Synonyms: TRADD; Monoclonal Antibody; TRADD (C-terminal region) Antibody; TRADD (C-terminal region); anti-TRADD antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M339
• Specificity: This antibody detects a 34kDa* protein corresponding to the apparent molecular mass of TRADD on SDS-PAGE immunoblots of adult mouse brain.
• Purity/Purification: Protein A chromatography
• Form/Format: Mouse monoclonal purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-TRADD antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:2000; ICC: 1:100; WB: 1:1000
• Immunogen: Clone M339 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human TRADD. This sequence has high homology to similar regions in rat and mouse TRADD.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal TRADD (C-terminal region) antibody at 1:250 (lane 1) or 1:1000 (lane 2).)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of TRADD in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with mouse monoclonal TRADD (C-terminal region) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight 594. The cells were counterstained with Sytox green to label nuclei.)

Related Product Information for anti-TRADD antibody

Tumor necrosis factor (TNF) activates TNF receptor-1 (TNFR1), leading to diverse biological activities in many cell types. TNFR1 contains an intracellular death domain that is required for signaling related to antiviral activity, programmed cell death, and NF-kappaB activation. TNFR1-associated death domain protein (TRADD) was isolated in a screen to identify proteins that interact with the death domain of TNFR1. TRADD is a 34-kDa protein that contains a 111-amino acid death domain with sequence similarity to TNFR1. This domain mediates oligomerization of TRADD and interaction with TNFR1. TRADD mRNA is expressed ubiquitously, and overexpression of TRADD causes TNF-induced apoptosis and activation of NF-kappaB. These responses require the TRADD death domain. In addition to TNF signaling, TRADD N-terminus can interact with Stat1 in response to IFNgamma, and this interaction can negatively regulate Stat activity.

References

Wesemann, D.R. et al. (2004) Nat. Immun. 5:199.
Hsu, H. et al. (1995) Cell 81:495.

NCBI and Uniprot Product Information

• NCBI GI #: 13378137
• NCBI GeneID: 8717
• NCBI Accession #: NP_003780.1
• NCBI GenBank Nucleotide #: NM_003789.3
• UniProt Accession #: Q15628; Q52NZ1; B2RDS3
• Molecular Weight: 34,247 Da
• NCBI Official Full Name: tumor necrosis factor receptor type 1-associated DEATH domain protein
• NCBI Official Synonym Full Names: TNFRSF1A-associated via death domain
• NCBI Official Symbol: TRADD
• NCBI Official Synonym Symbols: Hs.89862
• NCBI Protein Information: tumor necrosis factor receptor type 1-associated DEATH domain protein; TNFR1-associated death domain protein; tumor necrosis factor receptor-1-associated protein; tumor necrosis factor receptor type 1 associated death domain protein
• UniProt Protein Name: Tumor necrosis factor receptor type 1-associated DEATH domain protein
• Protein Family: Tumor necrosis factor receptor type 1-associated DEATH domain protein
• UniProt Gene Name: TRADD
• UniProt Synonym Gene Names: TNFR1-associated DEATH domain protein
• UniProt Entry Name: TRADD_HUMAN

NCBI Description

The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

TRADD: Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. Heterodimer with TNFRSF1A/TNFR1. Interacts with DAB2IP, FADD, HIPK2, KRT14, KRT16, KRT17, KRT18, RIPK1, SQSTM1, TRAF1, TRAF2 and TRPC4AP. Found in all examined tissues.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: cytoskeleton; cytoplasm; nucleus; cytosol; receptor complex; lipid raft

Molecular Function: identical protein binding; signal transducer activity; protein binding; protein complex binding; tumor necrosis factor receptor binding; kinase binding; molecular adaptor activity

Biological Process: caspase activation; positive regulation of hair follicle development; positive regulation of I-kappaB kinase/NF-kappaB cascade; induction of apoptosis via death domain receptors; tumor necrosis factor-mediated signaling pathway; protein heterooligomerization; apoptosis; positive regulation of apoptosis; signal transduction; activation of NF-kappaB transcription factor