Quality Control
(Western blot analysis of immunized recombinant protein, using anti-TPX2 monoclonal antibody. )
Mouse anti-Human TPX2 Monoclonal Antibody | anti-TPX2 antibody
Mouse monoclonal antibody Anti-Human TPX2
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-TPX2 monoclonal antibody. )
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-TPX2 monoclonal antibody. )
Quality Control #2
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control #2
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Western Blot (WB)
(Detection of human TPX2 by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 3164C6a-1]Predicted molecular weight: 85 kDa)
Western Blot (WB)
(Detection of human TPX2 by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 3164C6a-1]Predicted molecular weight: 85 kDa)
Immunoprecipitation (IP)
(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-TPX2 mAb. [Lot No. 3164C6a-1]Predicted molecular weight: 85 kDa)
Immunoprecipitation (IP)
(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-TPX2 mAb. [Lot No. 3164C6a-1]Predicted molecular weight: 85 kDa)
Flow Cytometry (FC/FACS)
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TPX2 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 3164C6a-1])
Flow Cytometry (FC/FACS)
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TPX2 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 3164C6a-1])
Immunocytochemistry (ICC)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TPX2 mAb. Nuclear was stained with Hoechst (blue fluorescence). [Lot No. 3164C6a-1])
Immunocytochemistry (ICC)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TPX2 mAb. Nuclear was stained with Hoechst (blue fluorescence). [Lot No. 3164C6a-1])
NCBI and Uniprot Product Information
Uniprot Description
TPX2: a microtubule-associated protein required for meiotic and mitotic spindle assembly. Targets a plus end-directed motor (XKLP2) to the minus ends of microtubules in the mitotic spindle. Required for microtubule nucleation around chromosomes in response to the local generation of RanGTP. Binds to and activates Aurora A kinase and targets it to the mitotic spindle.
Protein type: Microtubule-binding; Cell cycle regulation
Chromosomal Location of Human Ortholog: 20q11.2
Cellular Component: axon hillock; nucleoplasm; microtubule cytoskeleton; spindle pole; microtubule; cytoplasm; spindle; nucleus
Molecular Function: protein binding; GTP binding; protein kinase binding; ATP binding
Biological Process: cell proliferation; mitosis; apoptosis; cell division; activation of protein kinase activity
Research Articles on TPX2
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Product Notes
The TPX2 tpx2 (Catalog #AAA120232) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human TPX2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TPX2 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the TPX2 tpx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPX2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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