Mouse TPSAB1 / Mast Cell Tryptase Monoclonal Antibody | anti-TPSAB1 antibody

Mouse Monoclonal (IgG1) to Human TPSAB1 / Mast Cell Tryptase

Cat. Num. AAA245264

• Gene Names: TPSAB1; TPS1; TPS2; TPSB1
• Reactivity: Cat, Dog, Human, Monkey
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein G Purified
• Synonyms: TPSAB1 / Mast Cell Tryptase; Monoclonal Antibody; Mouse Monoclonal (IgG1) to Human TPSAB1 / Mast Cell Tryptase; Anti-TPSAB1 / Mast Cell Tryptase Antibody IHC-plus; TPSAB1; Alpha tryptase; Mast cell alpha II tryptase; Tryptase alpha-1; Tryptase beta I; Tryptase-III; TPSB1; Tryptase alpha II; TPS1; TPS2; Tryptase beta-1; Tryptase-1; Tryptase-I; Mast cell beta I tryptase; Tryptase alpha/beta 1; Human TPSAB1; Mast Cell Tryptase; anti-TPSAB1 antibody

• Host: Mouse
• Reactivity: Cat, Dog, Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Recognizes human mast cell tryptase, both alpha and beta isoforms. The antibody is an excellent marker for mast cells and does not bind to any other cell type in Immunohistochemistry. Species cross-reactivity: feline, canine and monkey.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.09% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 275

• Applicable Applications for anti-TPSAB1 antibody: Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-Fr, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Human mast cell tryptase purified from human lung tissue.
• Immunogen Type: Purified protein
• Immunogen: TPSAB1 / Mast Cell Tryptase antibody was raised against human mast cell tryptase purified from human lung tissue.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-TPSAB1 / Mast Cell Tryptase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Product Categories/Family for anti-TPSAB1 antibody

Family: Protease; Subfamily: Serine S1

NCBI and Uniprot Product Information

• NCBI GI #: 13775595
• NCBI GeneID: 7177
• NCBI Accession #: NP_003285.2
• NCBI GenBank Nucleotide #: NM_003294.3
• UniProt Accession #: Q15661; P15157; Q15663; Q6B052; Q9H2Y4; Q9H2Y5; Q9UQI1; D2E6R9; D2E6S1
• Molecular Weight: 29,533 Da
• NCBI Official Full Name: tryptase alpha/beta-1
• NCBI Official Synonym Full Names: tryptase alpha/beta 1
• NCBI Official Symbol: TPSAB1
• NCBI Official Synonym Symbols: TPS1; TPS2; TPSB1
• NCBI Protein Information: tryptase alpha/beta-1; tryptase I; tryptase-1; tryptase-I; tryptase-III; tryptase beta 1; tryptase beta I; tryptase beta-1; tryptase alpha-1; tryptase alpha II; mast cell beta I tryptase; mast cell alpha II tryptase
• UniProt Protein Name: Tryptase alpha/beta-1
• Protein Family: Tryptase
• UniProt Gene Name: TPSAB1
• UniProt Synonym Gene Names: TPS1; TPS2; TPSB1; Tryptase-1
• UniProt Entry Name: TRYB1_HUMAN

NCBI Description

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

TPSAB1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; EC 3.4.21.59; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response; proteolysis