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Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Recombinant Protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Protein Lane 2. : HepG2 cell lysate)

Mouse anti-Human TPMT Monoclonal Antibody | anti-TPMT antibody

TPMT antibody

Gene Names
TPMT; TPMTD
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
By protein-G affinity chromatography
Synonyms
TPMT; Monoclonal Antibody; TPMT antibody; Thiopurine S-methyltransferase; Thiopurine S-methyltransferase HGNC:12014; S adenosyl L methionine thiopurine S methyltransferase; Thiopurine methyltransferase; Thiopurine S methyltransferase.; anti-TPMT antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT2E7
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
245
Applicable Applications for anti-TPMT antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1,000
Antigen Species
Human
Immunogen
Recombinant human TPMT (1-245aa) purified from E. coli
Clone Note
Anti-human TPMT mAb, clone AT2E7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TPMT protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Recombinant Protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Protein Lane 2. : HepG2 cell lysate)

Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Recombinant Protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Protein Lane 2. : HepG2 cell lysate)

Immunofluorescence (IF)

(ICC/IF analysis of TPMT in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TPMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

Immunofluorescence (IF) (ICC/IF analysis of TPMT in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TPMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-TPMT antibody
TPMT (Thiopurine S-methyltransferase) is a very important enzyme for the metabolizm of thiopurine compounds. TPMT pharmacogenetics has been studied extensively because of its clinical significance. Many patients got benefited from the knowledge of TPMT genotype-phenotype correlation. Also, TPMT activity exhibits autosomal codominant genetic polymorphism, and patients inheriting TPMT-deficiency are at high risk of potentially fatal hematopoietic toxicity.
Product Categories/Family for anti-TPMT antibody
References
Milstein O., et al. (2008) J Biol Chem. 283 (49):34414-22.; Aldape MJ., et al. (2007) J Infect Dis. 195 (12):1838-45.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,180 Da
NCBI Official Full Name
thiopurine S-methyltransferase isoform 1
NCBI Official Synonym Full Names
thiopurine S-methyltransferase
NCBI Official Symbol
TPMT
NCBI Official Synonym Symbols
TPMTD
NCBI Protein Information
thiopurine S-methyltransferase
UniProt Protein Name
Thiopurine S-methyltransferase
UniProt Gene Name
TPMT

NCBI Description

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014]

Uniprot Description

Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.

Research Articles on TPMT

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Product Notes

The TPMT tpmt (Catalog #AAA200217) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TPMT antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TPMT can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:1,000. Researchers should empirically determine the suitability of the TPMT tpmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPMT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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