anti-Human Topoisomerase II Monoclonal Antibody

Anti - Topoisomerase II (DNA) alpha

Cat. Num. AAA606686

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: Topoisomerase II; Monoclonal Antibody; Anti - Topoisomerase II (DNA) alpha; Topoisomerase II (DNA) alpha; anti-Topoisomerase II antibody

• Host: Host: Mouse; Source: Human
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 7C52
• Specificity: Recognizes human Topoisomerase II (DNA) alpha. No significant reaction is seen with TOP2 beta. Species Sequence Homology: Guinea pig, porcine - 94%; Mouse - 87%; Chicken - 72%; Rat - 88%.
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Affinity Purified; Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
• Concentration: ~1mg/ml (varies by lot)
• Sequence Length: 184

• Applicable Applications for anti-Topoisomerase II antibody: ELISA, Western Blot
• Application Notes: Suitable for use in ELISA and Western Blot Other applications not tested.; Dilution: ELISA: 0.1-1ug/ml; Control peptide can be used to coat plates at 1ug/ml. See T8065-13G.; Western Blot: 2-10ug/ml is recommended for Western. Human Raji cells topoisomerase II alpha is ~170kD.; Optimal dilutions to be determined by the researcher.
• Immunogen: 18aa peptide from ~C-terminus of human Topoisomerase II alpha. Sequence is 100% conserved in alternatively spliced isoforms 2-4 of human TOP2A.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Topoisomerase II antibody

The topology of the DNA is a crucial issue in different biological processes like recombination, replication, transcription, and chromatin remolding. DNA topoisomerases are enzymes that control the amount of supercoiling in DNA. Without topoisomeras, DNA cannot replicate normally. The protein family of DNA topoisomerases is divided into two types (type I and II) based on their mechanism and physical properties. While the type I topoisomerases are monomeric and do not require ATP, the type II contain multi-subunits and depend on ATP for their activity. The type I enzymes include eukaryotic and bacterial topoisomerase I and III. The type II enzymes include bacterial DNA gyrase and topoisomerase IV and the eukaryotic topoisomerase II. Type I enzymes introduce transient single strand breaks in DNA, while type II enzymes produce transient double strand breaks in the DNA. Human DNA Topoisomerase I belongs to the subtype IB enzymes. The protein has four distinct domains. The N-terminal 214 amino acids are dispensable for relaxation activity and constitutes a hydrophilic and highly protease sensitive region of the protein. There are four nuclear localization signals in the N-terminal domain. The second domain is a core domain of 421 amino acids that is responsible for the single break in the DNA. Recently it was reported that DNA topoisomerase I is involved in modulation of RNA and is present in retroviral particles (like HIV1). Furthermore, the enzyme enhances HIV-1 cDNA production in reverse transcription assays.

Product Categories/Family for anti-Topoisomerase II antibody

Abs to Topoisomerases

References

• Jenkins JR et al (1992) Nucl. Acid Res. 20, 5587-5592; Austin CA et al (1993) BBA 1172, 283-291; Chung TD et al (1989) PNAS 86, 9431-9435; Sng JH et al (1999) BBA 1444, 395-406; Wang JC et al (1985) Ann Rev. Bicohem 54, 665-697; Earnshaw WC et al (1985) j Cell Biol. 100, 1706-1715

NCBI and Uniprot Product Information

• NCBI GI #: 162532534
• NCBI Official Full Name: topoisomerase II