Mouse anti-Human Topoisomerase I Monoclonal Antibody | anti-TOP1 antibody

Topoisomerase I (DNA Topoisomerase 1, DNA Topoisomerase I, NUP98 Fusion Gene, TOP 1, TOP1, TOP-1, TOP I, TOPI, TOP-I, Topoisomerase (DNA) I, Topoisomerase 1, Topoisomerase1, Topoisomerase-1, Topoisomerase-I, Type I DNA Topoisomerase) (Azide Free) (MaxLigh

Cat. Num. AAA6231171

• Gene Names: TOP1; TOPI
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: Topoisomerase I; Monoclonal Antibody; Topoisomerase I (DNA Topoisomerase 1; DNA Topoisomerase I; NUP98 Fusion Gene; TOP 1; TOP1; TOP-1; TOP I; TOPI; TOP-I; Topoisomerase (DNA) I; Topoisomerase 1; Topoisomerase1; Topoisomerase-1; Topoisomerase-I; Type I DNA Topoisomerase) (Azide Free) (MaxLigh; anti-TOP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 11C887
• Specificity: Recognizes human Topoisomerase I.
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-TOP1 antibody: Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant protein corresponding to a portion of amino acids within aa692-766 from human TOP1 (NP_003277) with GST tag. MW of GST tag alone is 26kD.
• Conjugate: MaxLight750
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-TOP1 antibody

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq]

Product Categories/Family for anti-TOP1 antibody

Antibodies; Topoisomerases

NCBI and Uniprot Product Information

• NCBI GI #: 11225260
• NCBI GeneID: 7150
• NCBI Accession #: NP_003277
• NCBI GenBank Nucleotide #: NM_003286
• UniProt Accession #: P11387
• Molecular Weight: 91kDa
• NCBI Official Full Name: DNA topoisomerase 1
• NCBI Official Synonym Full Names: DNA topoisomerase I
• NCBI Official Symbol: TOP1
• NCBI Official Synonym Symbols: TOPI
• NCBI Protein Information: DNA topoisomerase 1
• UniProt Protein Name: DNA topoisomerase 1
• Protein Family: Topoisomerase I damage affected protein
• UniProt Gene Name: TOP1
• UniProt Entry Name: TOP1_HUMAN

NCBI Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]

Uniprot Description

TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family.

Protein type: Oncoprotein; Nucleolus; Isomerase; EC 5.99.1.2

Chromosomal Location of Human Ortholog: 20q12-q13.1

Cellular Component: nucleoplasm; nuclear chromosome; nucleolus; perikaryon; nucleus

Molecular Function: DNA topoisomerase (ATP-hydrolyzing) activity; protein binding; DNA binding; chromatin binding; DNA topoisomerase type I activity

Biological Process: circadian rhythm; response to drug; chromatin remodeling; DNA topological change; viral reproduction; programmed cell death; circadian regulation of gene expression; DNA replication; phosphorylation; embryonic cleavage; chromosome segregation